应用噬菌体随机肽库研究抗溶藻弧菌及其外膜蛋白单抗识别的特异性表位  

Epitope Identification of Monoclonal Antibody Against Vibrio alginolyticus and Outer Membrane Proteins of Vibrio alginolyticus with Phage-displayed Random Peptide Library

作　　者：黄志坚[1] 何建国[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2006年第5期67-70,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家"863"计划基金资助项目(2001AA622020);广东省科技厅资助项目(A3050201)

摘　　要：用噬菌体随机七肽库筛选制备的抗溶藻弧菌及其外膜蛋白的纯化单抗,经3轮淘洗,获得较高程度富集的噬菌体,效价较高,第3轮淘洗比第1轮淘洗的产量高出近百倍。同时获得的阳性噬菌体经ELISA检测能与Va抗原发生强的阳性反应,确证了淘洗筛选的结果。A 7-mer random peptide library displayed on M13 phage was used for screening the epitope of monoclonal antibody against Vibrio alginolyticus and outer membrane proteins of Vibrio alginolyticus. Three rounds of biopanning were carried out and Enzyme Linked Immunosorbent Assay （FLIA） was used to estimating the enrichment. The phages with high titer of enrichment can be harvested. Yields of the third round biopanning were above one hundred times more than those of the first round biopanning. The selection result of biopanning was further ensured by ELISA using the positive phage and Va antigen. Detection results indicated that the positive phage can specifically bind to Va antigen. Further studies are being carried out to map antigen epitope using DNA sequencing and computer analysis.

关 键 词：噬菌体随机肽库 溶藻弧菌 外膜蛋白 单克隆抗体 表位 

分 类 号：S941[农业科学—水产养殖]