籼稻不育系中A完善再生系统的研究  

Efficient Plant Regeneration from in Vitro Culture of Indica Rice Zhong A (Oryza sativa L.)

作　　者：王亚琴[1] 梁承邺[2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510640 [2]中国科学院华南植物园,广东广州510650

出　　处：《中山大学学报（自然科学版）》2006年第5期81-84,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省国际合作资助项目(2004B50201021);广州市科技计划资助项目(2004J1-C0231)

摘　　要：以籼稻不育系中A的种子为外植体,探讨了培养基配方、外源激素、营养物等对其愈伤组织诱导和绿苗分化的影响。结果表明,在NB培养基中添加2.5 mg/L 2,4-D以及脯氨酸、水解酪蛋白、山梨醇等营养物有利于提高籼稻中A的愈伤诱导率,添加外源激素3.0 mg/L的6-BA和0.5 mg/L的NAA有利于提高其分化率,添加0.1 mg/L NAA可以使生根率达到100%。由此初步建立了籼稻中A的高效再生系统,为其遗传转化的顺利进行奠定了基础。Factors affecting the callus induction and shoot differentiation of indica rice Zhong A, such as media, exogenous hormones and nutrient substances, etc. , were investigated. The results showed that high induction frequency of mature seeds derived embryogenic calli was initiated on NB medium supplement with 2. 5 ～ 3.0 mg/L 2, 4-D and nutrient substances such as praline, casein acid hydrolysate and D-sorbitol, high differentiation frequency was obtained on NB medium supplement with 3.0 mg/L 6 - BA and 0. 5 mg/L NAA, and 100 % rooting frequency was obtained on NB medium supplement with 0. 1 mg/L NAA. Therefore a high efficient regeneration system from in vitro culture of indica rice Zhong A was established for genetic transformation.

关 键 词：籼稻中A 愈伤组织 组织培养 高效再生系统 

分 类 号：Q813[生物学—生物工程]