H_1型调宁蛋白mRNA在胆道中的表达与妊娠豚鼠胆囊结石形成关系的实验研究  

Experimental study on relationship between h_1-Calponin mRNA expression in biliary tract and gallstone formation in pregnant guinea pig

作　　者：汪斌[1] 丁佑铭[1] 王卫星[1] 余莉[1] 洛若愚[2] 程邦昌[3] 汪炳华[4] 

机构地区：[1]武汉大学人民医院肝胆腔镜外科 [2]武汉大学人民医院妇产科 [3]武汉大学人民医院胸外科 [4]武汉大学医学院生物化学与分子生物学教研室,湖北武汉430060

出　　处：《中国现代医学杂志》2006年第20期3061-3064,3068,共5页China Journal of Modern Medicine

基　　金：湖北省计划生育委员会科研基金资助(基金编号:301140240)

摘　　要：目的探讨妊娠胆囊结石形成过程中h1型调宁蛋白mRNA表达变化对胆道动力学改变的影响,从分子水平上阐明妊娠胆结石形成的机制。方法将30只成年雌性豚鼠随机等分为未孕组、孕30d组和孕60d组3组,每组各10只。实验组豚鼠按雌雄4∶1比例搭配同笼喂养交配,建立豚鼠妊娠胆结石模型。动物剖腹后穿刺胆囊收集胆汁;采集门静脉血,分离血清;取除胆囊及Oddi括约肌。分别测定胆汁中总胆固醇(TC)、总胆红素(TBiL)、总胆汁酸(TBA)浓度,血清雌二醇(E2)及孕酮(Pg)含量,应用半定量RT-PCR法测定胆囊平滑肌和Oddi括约肌中h1型调宁蛋白(h1-Calponin)mRNA的表达量变化。结果妊娠豚鼠胆汁呈成石性胆汁生化改变。孕60d组胆汁TC、TbiL较非孕组明显增高(P<0.05),TBA浓度显著降低(P<0.01);血清E2、Pg含量较非孕组明显升高(P<0.001);孕60d组豚鼠胆囊平滑肌中h1-calponinmRNA表达量明显高于非孕组(P<0.001),而Oddi括约肌中h1-calponinmRNA表达量较非孕组明显降低(P<0.01)。结论妊娠豚鼠h1-calponinmRNA表达在胆囊平滑肌中增高,在Oddi括约肌中下降,导致胆道运动功能紊乱和胆汁淤滞,可能是妊娠胆囊结石形成的关键因素。[Objective] To explore effects of the expression of h1-Calponin mRNA on the biliary tract dynamics and investigate the molecular mechanisms of gallstone formation in pregnancy. [Methods] 30 femal guinea pigs were divided randomly into 3 groups, the nonpregnant group （n=10）, the 30 days of pregnancy （n=10） and the 60 days of pregnancy group （n=10）. Animal models of pregnancy were established on experimental guinea pigs through feeding animals with one cage according to female versus male as 4：1. The bile,the portal vein blood, the gallbladder smooth muscles and Oddi sphincters （OS） were obtained respectively. The concentration of total cholesterol （TC）, total bilirubin （TBiL） and total bile acid （TBA） in the bile, the serum estradiol （E2） and progesterone （Pg） levels were determined respectively. Expression levels of h1-Calponin mRNA in gallbladder smooth muscles and Oddi sphincters （OS） were evaluated by semiquantitative reverse tanscription polymerase chain reaction（RT-PCR）. [Results] The concentration of TC and TBiL were signifieanlly more increased in the 60 days of pregnancy than that in nonpregnant group （P 〈0.05）, however, that of TBA decreased （P 〈0.01）, while that of the serum E2 and Pg in pregnant group were higher than that in nonpregnant group （P 〈0.001）. Up-regulation of h1-Calponin gene expression was observed in gallbladder smooth muscles in the 60 days of pregnancy, but converse in 0S. [Conclusion] Up-regtdation of h1-Calponin gene expression in gallbladder smooth muscles and down-regulation in OS might attribute to induce dysfunction of extrahepatic biliary tract, bile stasis and gallstone formation in pregnancy.

关 键 词：基因表达 收缩蛋白质类 胆囊结石 妊娠 豚鼠 

分 类 号：R657.42[医药卫生—外科学]