沙眼衣原体15个血清型omp1基因的VS1和VS2区序列分析  

Nucleotide acid sequences for VS1 and VS2 regions of the omp1 gene of the 15 Chlarmydia trachomatis serovars

作　　者：郑和平[1] 江丽芳[1] 薛耀华[2] 方丹云[1] 冯占芹[2] 吴亚安[2] 黄进梅[2] 

机构地区：[1]中山大学医学院,广东广州510089 [2]广东省皮肤性病防治中心,广东广州510500

出　　处：《中国微生态学杂志》2006年第5期365-366,368,共3页Chinese Journal of Microecology

基　　金：广东省科委资助项目(2005B34201011);广东省卫生厅资助项目(A2005150)

摘　　要：目的比较分析沙眼衣原体15个血清型omp1基因VS1和VS2序列的同源性和变异性。方法巢式PCR扩增VS1-VS2基因，自动测序仪测定核苷酸序列，DNAstar生物软件进行比对分析。结果15个血清型沙眼衣原体扩增出大小453bp的VS1-VS2基因。VS1区域序列比对显示血清群B和中间群的VS1核苷酸序列相对保守，而血清群C各型VS1区域表现出较大的核苷酸变异，型间显示1～9个核苷酸替换，且发生在中心区域。血清型VS2序列较VS1存在更多的变异，血清群B中各血清型间均存在2～19个核苷酸的改变，血清群C表现为4～8个核苷酸差异，中间群的F和G型之间存在6个核苷酸差异。结论阐明VS1和VS2区核苷酸的多态性，为下一步进行该蛋白表达和构建寡核苷酸型特异性探针奠定了基础。Objective To compare the identity and divergence of VS1 and VS2 regions of the ompl gene between 15 Chlarmydia trachomatis serovars. Methods The VS1-VS2 was amplified by a nested PCR and sequenced by a sequencer. Identity and divergence of VS1 and VS2 were analyzed using DNAstar software. Results 516 bp fragments of VS1-VS2 were amplified from 15 serovars. Compared with group B and intermediate, VDI of group C shows more divergence with 1～9 substitution between 15 serovars. In contrast to VS1, VS2 was found to be more variability. 2～9 nucleotide were changed among group B,4～8 among group C and 6 between serovar F and G. Conelusions Heterogeneity of VS1 and VS2 between 15 serovars was illuminated. The findings should be useful for further expressing the protein and constructing oligonucleotide probes.

关 键 词：沙眼衣原体 血清型 omp1基因 

分 类 号：R374.1[医药卫生—病原生物学]