ICU患者脑膜炎败血黄杆菌的耐药性分析和研究  

Analyzing and reserching of drug resistance about Chryseobacterium meningosepticum from ICU patients' specimen of sputum

作　　者：王冬国[1] 王红戟 徐昌君[1] 

机构地区：[1]台州市立医院,浙江台州318000 [2]台州市路桥区疾病控制中心

出　　处：《中国微生态学杂志》2006年第5期378-379,383,共3页Chinese Journal of Microecology

摘　　要：目的检测从ICU患者痰液标本中分离的产MBL、ESBL脑膜炎败血黄杆菌的耐药性状况,指导临床正确合理使用抗生素。方法用VITEK32鉴定菌株;双纸片协同试验筛选产MBL菌株、产ESBL菌株;药敏试验使用VITEK GNS143上机检测和K-B法检测加做的药敏试验。结果ICU患者痰液标本中的脑膜炎败血黄杆菌的产酶率为78.4%,其中产MBL为41.2%,产ESBL为29.4%,有4株同时产MBL和ESBL。美满霉素、万古霉素、力奈唑烷的耐药率分别为19.8%、1.98%、1.98%。结论脑膜炎败血黄杆菌在ICU患者的痰液标本中高耐药性的根本原因是高产酶率(高产MBL和ESBL)。万古霉素、力奈唑烷、美满霉素是治疗ICU患者呼吸道感染的最有效药物。Objective To investigate drug resistance of Chryseobacterium meningosepticum which producing metallo-β-lactamase and extended-spectrum-β-lactamase from ICU patients＇ specimen of sputum so as to guide the rational application of antibiotics. Methods Identified the strains with VITEK 32 ; MBL was screened by double disk synergy and ESBL was screened by double disk synergy; Antimicrobial sensitivity of clinical isolated was tested by VITEK GNS143 and adding antimicrobial sensitivity was tested by Kirby-Bauer method. Results The rate of producing β-lactamase of Chryseobacterium meningosepticum was 78.4%,and the rate of producing MBL was 41.2%, the rate of producing ESBL was 29. 4%,respectivily, four clinical isolates produced both MBL and ESBL. The resistance rate of Chryseobacterium meningosepticum clinical isolates to Minocycline, Vancomycin, Linezolid was 19.8%, 1.98%,1.98%. Conclusions Why the high resistance rate of Chryseobacterium meningosepticum in ICU patients＇ specimen of sputum was that high producing β-lactamase（high producing MBL and ESBL）. Vancomycin, Linezolid, Minocycline were the most effective agents for treatment in ICU patients＇ specimen of sputum.

关 键 词：ICU患者 脑膜炎败血黄杆菌 MBL ESBL 耐药率 

分 类 号：R378[医药卫生—病原生物学]