凝固酶阴性葡萄球菌菌谱与药敏谱调查  

Investigation of distribution and antimicrobial resistance in coagulase negative Staphylococcus

作　　者：张永标[1] 梁彩倩[1] 张扣兴[2] 赵锋 席云[3] 文博[3] 

机构地区：[1]中山大学附属第三医院急诊科,广东广州510630 [2]中山大学附属第三医院呼吸科,广东广州510630 [3]中山大学附属第三医院检验科,广东广州510630

出　　处：《中国微生态学杂志》2006年第5期395-396,共2页Chinese Journal of Microecology

摘　　要：目的了解中山大学附属第三医院临床分离凝固酶阴性葡萄球菌(CNS)的菌种构成与耐药性。方法采用MicroScan WalkAway4-0全自动微生物鉴定仪及配套阳性菌药敏复合板进行菌种鉴定与药敏试验,ni-trocefin法测定β-内酰胺酶。结果表皮葡萄球菌与溶血葡萄球菌共占CNS的70.4%;甲氧西林耐药株(MRCNS)与甲氧西林敏感株(MSCNS)各占77.8%、22.2%;药敏试验结果提示MRCNS仅对万古霉素、呋喃妥因敏感,MSCNS对大多数抗生素敏感,未检出万古霉素耐药与中介株;β-内酰胺酶在MRCNS、MSCNS中的检出率分别为91.7%、78.1%。结论临床分离的CNS以表皮葡萄球菌和溶血葡萄球菌为主,MRCNS多重耐药现象严重,宜密切监测CNS的分离状况与耐药性,并严格限制万古霉素在临床上的应用,以避免耐万古霉素CNS的出现与流行。Objective To investigate the distribution and antimicrobial resistance of coagulase negative Staphylococcus （CNS） clinical isolates. Method Bacterial confirmatory and antibiotic susceptibility tests were carried out by Microscan WalkAway-40 system and Positive Combo Panel Type ll,the phenotypes of CNS strains harboring β-lactamases were detected by nitrocefin method. Results Staphylococcus epidermidis and Staphylococcus haernolyticus accounted for 70. 4% of all CNS; Methicillin-resistant （MRCNS） and methicillin-sensitive （MSCNS） strains accounted for 77.8% and 22.2% of CNS respectively. Antibiotic susceptibility tests indicated that MRCNS were sensitive only to vancomycin and nitrofurantoin, while MSCNS to most antibiotics. No vancomycin-resistant orintermediate strains were found. β-lactamases producing strains were detected in 91.7% of MRCNS and 78. 1% of MSCNS. Conclusions Staphylococcus epidermidis and Staphylococcus haemolyticus are the major microorganisms in CNS clinical isolates. The situation of multidrug resistance in MRCNS is serious. The distribution and antimicrobial resistance of CNS should be monitored closely, and the use of vancomycin should be restricted, so as to avoid emersion and prevalence of vancomycin-resistant CNS in clinic.

关 键 词：凝固酶阴性葡萄球菌 耐药性 抗菌药物 

分 类 号：R378.1[医药卫生—病原生物学]