人类疱疹病毒7型糖蛋白(gL)的研究  

The Preliminary Exploration of Glycoprotein L of Human Hepersvirus 7

作　　者：窦洁[1] 姚堃[1] 徐建[1] 陈云[1] 周峰[1] 彭光勇[1] 

机构地区：[1]南京医科大学基础医学院微生物与免疫学教研室,江苏南京210029

出　　处：《药物生物技术》2006年第5期326-329,共4页Pharmaceutical Biotechnology

基　　金：国家自然科学基金资助项目编号No30371345;江苏省自然科学基金资助项目BK2001115

摘　　要：克隆人类疱疹病毒7型(HHV-7)糖蛋白gL,分别连接入酵母双杂交载体pAS2-1、pACT2。通过双杂交技术,分析gL基因表达蛋白与gH、CD4的相互作用。发现含gL、gH基因的重组酵母菌Y190具有β-半乳糖苷酶活性,而含gL、CD4基因的重组Y190未检测到相应活性。结果表明,gL表达蛋白在酵母双杂交系统中能够与另一HHV-7糖蛋白gH结合,而与HHV-7已知受体CD4无相互作用。Gly coprotein DNA from HHV-7 infected SupT1 cells was cloned and then inserted into the yeast two-hybrid vectors pAS2-1, pACT2 to study its relation with gH and CD4. In yeast two-hybrid system, β-galactosidase activity was detected in the yeast Y190 containing HHV-7 gL and gH, but not in the Y190 containing gL and CD4. The results showed that HHV7 gL and gH protein can directly combine each other but no direct relations between gL and CD4, were found.

关 键 词：人类疱疹病毒7型 糖蛋白L 糖蛋白H 白细胞分化抗原4 酵母双杂交 

分 类 号：Q51[生物学—生物化学]