套式PCR检测蚊体内疟原虫的研究  

Detection of Plasmodium sporozoites in mosquitoes by nested-PCR

作　　者：周华云[1] 朱国鼎[1] 金小林[1] 李菊林[1] 王伟明[1] 曹俊[1] 顾亚萍[1] 高琪[1] 

机构地区：[1]江苏省寄生虫病防治研究所,无锡214064

出　　处：《中国血吸虫病防治杂志》2006年第5期365-368,共4页Chinese Journal of Schistosomiasis Control

基　　金："十五"国家科技攻关项目(2004BA718B13)

摘　　要：目的建立一种敏感、特异而快速的检测蚊体内疟原虫的方法。方法采用套式PCR方法,对人工感染间日疟原虫的嗜人按蚊、感染恶性疟和混合感染间日疟与恶性疟的大劣按蚊以及流行季节现场捕获的中华按蚊体内的疟原虫进行检测。结果28批人工感染间日疟原虫的嗜人按蚊、2批人工感染恶性疟的大劣按蚊和1批混合感染间日疟与恶性疟的大劣按蚊的检测结果与镜检结果符合率为100%;589只现场捕获的中华按蚊中,发现间日疟原虫阳性2只,阳性率为0.34%。结论本方法能快速而敏感地检出蚊体内不同种疟原虫。Objective To establish a fast and sensitive method to detect Plasmodium sporozoites in mosquitoes. Methods Anopheles anthropophagus （An. a） artificially infected with Plasmodium vivax （P. v）, Anopheles dirus （An. d） infected with Plasmodium falciparum （P. f） and both of P. v and P. f, field mosquitoes of Anopheles sinensis （An. s） captured in epidemic seasons were detected by nested-PCR. Results The results of 28 batches of An. a infected with P. v, 2 batches of An. d with P. f and 1 batch of An. d mixed with P. v and P. f by nested-PCR were accorded with the microscopical examination absolutely. Two positives of 589 An. s field mosquitoes were diseov： ered with a positive rate of 0.34%. Conclusion The nested-PCR is fast and sensitive for detecting different species of Plasmodium in mosquitoes.

关 键 词：PCR 嗜人按蚊 大劣按蚊 中华按蚊 疟原虫 

分 类 号：R382.31[医药卫生—医学寄生虫学]