淫羊藿甙、菟丝子提取物对雄激素部分缺乏大鼠生殖保护作用的比较研究  

Comparative studies on protective effects on reproduction of Icariin and the substance extracted from the semen cuscutae in rats with partial androgen deficiency

作　　者：章振保[1] 杨庆涛[1] 杨镜秋[1] 郑俊鸿[1] 周德荣[1] 

机构地区：[1]汕头大学医学院第二附属医院泌尿外科,广东汕头515041

出　　处：《中国老年学杂志》2006年第10期1389-1391,共3页Chinese Journal of Gerontology

摘　　要：目的探讨淫羊藿甙、菟丝子提取物对雄激素部分缺乏大鼠的生殖保护作用。方法以环磷酰胺造成生殖系统受损、雄激素部分缺乏大鼠模型,灌胃淫羊藿甙和菟丝子提取物7 d后,检测血清睾酮、黄体生成素(LH)、卵泡刺激素(FSH)含量;以HE染色和原位末端标记法(TUNEL)检测,光镜下观察睾丸组织、阴茎海绵体平滑肌细胞变化情况。结果①血清睾酮水平:淫羊藿甙组明显高于空白组和模型组,但低于睾酮组(P<0.05);菟丝子提取物组高于模型组,低于睾酮组(P<0.05),但与空白组间无统计学差异。②血清LH、FSH水平各组间均未见明显统计学差异。③阴茎海绵体平滑肌细胞的凋亡率:睾酮组和淫羊藿甙组明显低于模型组(P<0.05);菟丝子组与模型组则未见明显统计学差异。结论淫羊藿甙、菟丝子提取物对雄激素部分缺乏大鼠均具有生殖保护作用,但淫羊藿甙作用效果强于菟丝子提取物。Objective To evaluate the protective effects on reproduction of Icariin（ICA） and semen cuscutae（SSC） on partial androgen deficiency（PAD） rats. Methods The male rat model for impaired reproduction system and partial androgen deficiency were made by cyclophosphamide to determine the content of serum testosterone, luteinizing hormone （LH） and follicle-stimulating hormone （FSH） after ig. ICA and SCC. The change of histology of the testis and the penis were observed by microscope with the method of H-E staining and TUNEL method respectively. Results ①The contents of serum testosterone in ICA group were statistically higher than that in control group and model group, and were statistically lower than that in testosterone group （ P 〈 0. 05 ）. However, contents of serum testosterone in SSC group were higher than that in model group, and lower than that in testosterone group. But there had no statistically difference between SSC group and control group. ②There had no statistically difference among every group as to the contents of LH and FSH. ③The cell apoptosis rate in rat penis in testosterone group and ICA group were statistically lower than that in model group （P 〈 0. 05 ）. But there had no statisticaldifference between SSC group and model group. Conclusions ICA and SSC both have the protective effects on reproduction on partial androgen deficiency rats,but ICA is much more beneficial to PAD in the aging male （PADAM）.

关 键 词：淫羊藿甙 菟丝子提取物 雄激素部分缺乏 睾酮 大鼠 

分 类 号：R697.22[医药卫生—泌尿科学]