APOB基因XbaI多态性与中国维吾尔族自然长寿关联研究  

Investigation into the correlation of the polymorphism of APOB gene XbaI with the natural longevity in Uighur nationality

作　　者：姜文锡[1] 邱长春[2] 牛文全[2] 程祖亨[1] 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830054 [2]中国医学科学院基础医学研究所中国协和医科大学基础医学院医学分子生物学国家重点实验室,北京100005

出　　处：《中国热带医学》2006年第11期1928-1930,1959,共4页China Tropical Medicine

基　　金：国家自然科学基金(30160089);美国中华医学基金(CMB96-657)

摘　　要：目的探讨栽脂蛋白B（Apolipoprotein B，apoB）基因XbaⅠ多态性与维吾尔族自然长寿之阃的关系。方法选择新疆和田地区191名年龄90岁以上的健康维吾尔族个体为研究对象，另选53名年龄65—70岁、民族、性别、地域相匹配的正常个体为对照。采用PCR-序列特异引物（Sequence specifc primer，PCR—SSP）、PCR-限制长度片段多态性（Restriction fragmem length polymorphism，PCR—RFLP）和PCR-直接测序（PCR—sequencing）等技术对apoB基因第26外显子XbaⅠ限制性片段长度多态性（Restriction fragment length polymorphisms，XbaⅠ—RFLP）进行分型。结果经Logistic回归分析显示，长寿组apoB基因X^＋X^＋基因型频率显著高于对照组（P〈0．05）。结论基因型X^＋X^＋与自然长寿显著正相关，可能为长寿的保护因素。Objective To investigate the relationship between polymorphisms of the apolipoprotein B（apoB） gene XbaⅠ restriction fragment length polymorphisms（XbaⅠ- RFLP） and natural longevity in the Uygur ethinic group of Xinjiang Uygur Autonomous Region. Methods 191 healthy individuals over 90 years old and 53 individuals aged 65 - 70 years in Hetian Region were selected, The 26th exon of apoB gene XhaⅠ restriction fragment length polymorphisms was genotyped by using PCR - SSP, PCR - RFLP and PCR - sequencing. Results Logistic regression analyses revealed that the frequency of X^＋X^＋ genotype was significantly higher in longevity group compared to their controls. Conclusion Positive correlation is observed between X^＋X^＋ genotype and natural longevity in this study.

关 键 词：APOB基因 XbaⅠ 多态性 自然长寿 维吾尔族 

分 类 号：R29[医药卫生—民族医学]