硝苯地平对人牙龈成纤维细胞内Ca^(2+)含量影响的激光共聚焦显微观察  

Effect of Nifedipine on the Concentration of Intracellular Ca^(2+) in Gingival Fibroblast under Confocal Laser Microscopy

作　　者：李谨[1] 蒋春梅[1] 徐艳[1] 孙卫斌[1] 吴大明[1] 马骏驰[1] 胡凡[2] 

机构地区：[1]南京医科大学附属口腔医院口腔内科,江苏南京210029 [2]南京医科大学激光共聚焦室

出　　处：《口腔医学研究》2006年第5期495-497,共3页Journal of Oral Science Research

基　　金：南京医科大学科技发展基金(编号:NY2001011)

摘　　要：目的:探讨硝苯地平对牙龈成纤维细胞内Ca2+含量的影响。方法:体外培养人牙龈成纤维细胞,钙离子指示剂F luo-3染色,激光扫描共聚焦显微镜下观察加入不同浓度硝苯地平(NIF终浓度分别为1200、32.4 ng/mL)后荧光亮度的变化。结果:硝苯地平能抑制DHP类钙通道激动剂Bay K8644引起的牙龈成纤维细胞内钙离子含量增加(P<0.05),不同浓度硝苯地平的作用之间没有剂量-效应关系。结论:硝苯地平引起牙龈增生可能与抑制牙龈成纤维细胞内Ca2+含量有关。Objective： To investigate the effects of nifedipine on concentrations of Ca^2＋ in gingival fibroblast cells. Methotis： Gingival fibroblast cells were cultured in vitro, then dyed with Fluo -3. The changes of light density of cells under confocal laser microscopy were investigated. Results ： Nifedipine can inhabit the increase of concentration of Ca^2＋ in gingival fibroblast cells induced by Bay K8644 （ P 〈 0.05 ） , and this effect is independent to concentration of Nifedipine. Conelusion： Gingival hyperplasia induced by nifedipine may be related with the inhibition of fibroblast intracellular Ca^2＋ concentration.

关 键 词：硝苯地平 牙龈成纤维细胞 细胞内钙离子 

分 类 号：R96[医药卫生—药理学]