贵州省三都水族DYS287和DYS388基因座多态性分析  

An Analysis on Polymorphism of DNA Sites DYS287 and DYS388 in Shui Population of Guizhou,China

作　　者：何燕[1] 单可人[1] 吴昌学[1] 谢渊[1] 李毅[1] 官志忠[1] 任锡麟[1] 

机构地区：[1]贵州省医学分子生物学重点实验室

出　　处：《贵阳医学院学报》2006年第5期400-402,405,共4页Journal of Guiyang Medical College

基　　金：贵州省优秀科技人才省长专项基金资助项目[黔科教办(2001)3];贵州省科技厅基金资助项目[黔科合(2004)3007]

摘　　要：目的:调查Y染色体短串联重复序列(short tandem repeat,STR)DYS388基因座及Y染色体特异的Alu序列插入基因座DYS287的遗传多态在贵州省三都水族群体中的多态性分布。方法:采用聚合酶链反应及荧光标记基因扫描技术,分别对DYS287和DYS388基因座的遗传多态性进行分析。结果:在94名无关水族男性个体中,DYS287全部显示为YAP-,DYS388基因座共检出4种等位基因,DYS388×12基因频率高达0.9681。结论:获得贵州三都水族群体DYS287和DYS388基因座的遗传学数据,为该民族遗传关系的分析、法医学鉴定及该民族的起源提供了一定的遗传背景资料。Objective： To investigate the genetic polymorphism of chromosome Y specific short tandem repeat （Y-specific STR） locus DYS388 and the Y-chromosome specific Alu insert sequence DYS287 of Shui population in Shandu, Guizhou, China. Methods： DYS287 were amplified by PCR with special primers. DYS388 were amplified with fluorescently labeled primers, and genotypes were determined by ABI 377 Genetic Analyzer. Results： Among 94 unrelated males, 4 alleles were observed in locus DYS388. The allele frequency of DYS388 × 12 was 0.968 1. In all samples, YAP element was absent. Conclusion： The genetic data of DYS287 and DYS388 loci in Shai population have been obtained, and they might be useful in studies of population origin, relationship between populations, and forensic applications.

关 键 词：基因频率 多态现象(遗传学) Y染色体 聚合酶链反应 少数民族 贵州 短串联重复序列 

分 类 号：Q347[生物学—遗传学]