全生物化组织工程血管SMA、PDGF-AA及MMP-2的表达  

EXPRESSION OF ASMA,PDGF-AA AND MMP-2 IN THE COMPLETE BIOLOGICAL TISSUE ENGINEERED BLOOD VESSEL

作　　者：刘太华[1] 张炎[1] 姜宗来[2] 李玉泉[1] 刘波[2] 张秀花[1] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]上海交通大学医学院,上海200030

出　　处：《中国组织化学与细胞化学杂志》2006年第5期563-567,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金(10272110;10132020)

摘　　要：目的探讨血管平滑肌细胞的α-actin(SMA)、血小板源性生长因子-AA(PDGF-AA)及金属蛋白酶-2(MMP-2)的表达情况。方法采用以酶消化为主的方法制备猪颈总动脉脱细胞支架,再种植犬胸主动脉的平滑肌细胞,培养4周,分别于2周和4周取材作形态学观察和SMA、MMP-2及PDGF-AA的免疫组化染色及图象分析,并以幼年犬和成年犬的胸主动脉作对照。结果组织工程血管培养2周和4周,α-actin的表达高于幼年犬,但明显低于成年犬;PDGF-AA表达低于幼年犬,但明显高于成年犬;MMP-2高于成年犬,培养4周时MMP-2的表达接近幼年犬。结论全生物化组织工程血管体外构建中VSMC的PDGF-AA和ECM的表达逐渐增多,SMA的表达逐渐减少,VSMC由收缩表型逐渐转变为合成表型,VSMC大量增殖,伴有新的细胞外基质产生,同时MMP-2分泌增加,促进种植细胞的迁移,重建了组织工程血管的管壁结构。Objective To investigate the effect of α-actin （SMA）, PDGF-AA and MMP-2 from vascular smooth cells （VSMC） on reconstructing complete biological tissue engineered blood vessels （CBTEBV） in vitro. Methods The decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion. The VSMC isolated from dog thoracic aorta were seeded onto the inner surface of the scaffolds which then were cultured in vitro for 4 weaks. The case of reconstruction was checked by histological staining and electron microscopy. Immunohistochemical staining and image analysis were used to detect the expression of smooth muscle alphaactin （SMA）, platelet-derived growth factor-AA （PDGF- AA） and metalloproteinase-2 （MMP-2） in CBTEBV. Control groups included thoracic aortae of both immature dogs and adult dogs. Results The expressions of SMA was higher in CBTEBV cultured 2 or 4 weeks than that of immature dogs and significantly lower than that of the adult dogs. The expressions of PDGF-AA and extra cellular matrix （ECM） were progressively more. PDGF-AA was lower in the CBTE- BV cultured 2 or 4 weeks than that of the immature dogs and significantly higher than adult dogs. The expression of MMP-2 was higher in the CBTEBV cultured 2 or 4 weeks than that of the adult dogs, bat in CBTEBV cultured 4 weeks similar to that of the immature dogs. Conclusion Expressing progressively more PDGF-AA and ECM and less SMA, VSMC in CBTEBV transformed from the contractile phenotype to the synthetic phenotype, and proliferated rapidly during remodeling. At the same time, VSMC secreted increasingly more MMP-2 and migrated into scaffolds, resulting in the CBTEBV wall remodeling.

关 键 词：组织工程 血管重建 血管平滑肌细胞 血管平滑肌肌动蛋白 血小板源性生长因子 金属蛋白酶 

分 类 号：R318.11[医药卫生—生物医学工程]