重组人粒细胞集落刺激因子对药物性皮肤溃疡愈合的影响(英文)  

Recombinant human granulocyte-macrophage colony stimulating factor in the treatment of drug induced skin ulcer

作　　者：遆新宇[1] 刘颖格[1] 史皆然[1] 陈卫强[1] 赵峰[1] 吴昌归[1] 

机构地区：[1]解放军第四军医大学西京医院呼吸内科,陕西省西安市710032

出　　处：《中国临床康复》2006年第41期208-209,I0006,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:粒细胞集落刺激因子对成纤维细胞、角质细胞、皮肤黏膜细胞均有不同程度的刺激作用。目的:观察粒细胞集落刺激因子对药物性外渗所致皮肤溃疡的愈合作用。设计:随机对照动物实验。单位:解放军第四军医大学西京医院呼吸内科。材料:实验于2004-06/2004-11在解放军第四军医大学西京医院呼吸内科实验室完成。取雄性昆明种小白鼠20只,体质量18～24g。方法:将制备好的皮肤溃疡动物模型随机分为对照组和治疗组,每组10只。对照组给予酚妥拉明1mg,利多卡因20mg,地塞米松1mg,用生理盐水稀释至0.5mL,封闭,1次/d,共7d;治疗组在溃疡周围注射粒细胞集落刺激因子25μg,用生理盐水稀释至0.5mL,隔日给药1次,共7d。观察溃疡处皮肤组织的愈合时间和组织学变化。主要观察指标:观察粒细胞集落刺激因子对药物性外渗所致皮肤溃疡、糜烂的愈合时间和组织学变化。结果:20只小白鼠均进入结果分析。①愈合时间:对照组糜烂、溃疡的愈合时间明显长于治疗组[(20～24,8～12)d,t=2.264,P=0.01];②组织学观察:对照组治疗7d后肉芽组织增生不明显;治疗组治疗7d后肉芽组织增生,新生血管丰富。结论:粒细胞集落刺激因子能促进药物性糜烂、溃疡的创伤愈合。BACKGROUND： Recombinant human granulocyte-maerophage eolony stimulating factor （rhGM-CSF） could stimulate the proliferation of fibroblasts, keratinocyte and skin mueosae cells to different degrees. OBJECTIVE： To observe the effect of rhGM-CSF on the healing of drug exosmose induced skin ulcers. DESIGN： A randomized and controlled animal experiment. SETTING： Department of Respiratory Medicine, Xijing Hospital. Fourth Military Medical University of Chinese PLA. MATERIALS： This experiment was carried out at the Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University of Chinese PLA from June to November 2004. Totally 20 male Kunming white mice, with body mass of 18 to 24 g, were chosen. METHODS： Prepared skin ulcers animal models were randomly divided into control group and treated group with 10 white mice in each group. Mice in the control group were given ling phentolamine,20 mg lidocaine and ling dexamethasone diluted by normal saline to 0.5 mL , then sealed up , once a day for 7 days： 25 μg rhGM-CSF was diluted by normal saline to 0.5 ml, , then the solution was injected into the periphery of ulcers of mice in treated group , once every other day, for 7 days. Healing time and histological change of skin tissue at ulcer were observed. MAIN OUTCOME MEASURES： To observe the effect of rhGM-CSF on the healing time of drug exosmose induced skin ulcer and anabrosis and histological change RESULTS： Totally 20 mice entered the stage of result analysis. （1）Healing time ： the healing time of ulcer and erosion was significantly longer in control group than in treated group [（20-24,8-12）d.t=2.264,P=0.01];（2） Histological observation： hyperplasia of granulation tissue was not obviously on 7 days after treatment in control group; Hyperplasia of granulation tissue appeared and the newly born blood vessel was abundant on 7 days after treatment in the treated group . CONCLUSION： rhGM-CSF can promote the wound healing of drug induced anabrosis

关 键 词：粒细胞集落刺激因子/治疗应用 皮肤溃疡/药物疗法 疾病模型 动物 

分 类 号：R318[医药卫生—生物医学工程]