组胺对肺上皮细胞Toll样受体表达的上调作用  

Up-regulatory effect of histamine on Toll-like receptor expression in pulmonary epithelia cells

作　　者：侯一峰[1] 周艳春[1] 郑晓璇[1] 王海燕[1] 傅意玲[1] 方泽曼[1] 何韶衡[1] 

机构地区：[1]汕头大学医学院变态反应与炎症学研究所,广东汕头515041

出　　处：《第四军医大学学报》2006年第20期1867-1870,共4页Journal of the Fourth Military Medical University

基　　金：广东省自然科学基金重点项目(04106122)

摘　　要：目的：观察组胺对肺上皮细胞中Toll样受体（TLR）表达的影响．方法：体外培养人肺上皮细胞株A549，用RT-PCR和实时定量PCR检测静息状态或组胺作用下的A549细胞中TLR1～TLR10mRNA的变化，并用流式细胞术进一步确定组胺对TLR3表达的调节作用．结果：A549细胞有TLR1～TLR10 mRNA的组成型表达，组胺对TLR3 mRNA和蛋白的上调作用具有时间和剂量依赖性，并且H1受体阻断剂可抑制组胺对TLR3的上调作用．结论：组胺可上调肺上皮细胞中TLR3的表达，提示当呼吸道存在变态反应性病变时，增多的组胺可能会增加肺上皮细胞对病毒感染的敏感性．AIM： To investigate the up-regulatory effect of histamine on Toll-like receptor （TLR） expression in pulmonary epithelia cells. METHODS： TLR1-TLR10 mRNA expressions were determined by RT-PCR in resting A549 cells, and by real-time quantitive PCR in the cells following stimulation with histamine at various concentrations and at various periods of time. Furthermore, the up-regulatory effect of histamine on TLR3 was examined by flow cytometry. RESULTS ： TLR1-TLR10 mRNA were constitutively expressed in A549 cells. Histamine significantly enhanced TLR3 expression at both mRNA and protein levels in a concentration- and time-dependent manner. This effect of histamine was blocked by the H1 receptor antagonist. CONCLUSION： TLR3 expression can be up-regulated by histamine in A549 cells, which suggests that excessive histamine can enhance the sensitivity of pulmonary epithelium to virus infection when the allergic diseases exist in the respiratory tract.

关 键 词：组胺 肺/细胞学 上皮细胞 TOLL样受体 

分 类 号：Q291[生物学—细胞生物学]