一种新的人绒毛膜促性腺素压电免疫传感器  

A new piezoelectric quartz crystal immunosensor for hCG detection

作　　者：符婷[1] 王桦[1] 沈国励[1] 俞汝勤[1] 

机构地区：[1]化学生物传感与计量学国家重点实验室,化学化工学院,湖南大学,长沙410082

出　　处：《化学传感器》2006年第2期14-17,共4页Chemical Sensors

摘　　要：在石英晶体金电极上,通过半胱胺、羧基化的碳纳米管(CNTs)固定鼠抗人绒毛膜促性腺激素β-hCG抗体构建一种新的石英晶体压电免疫传感器,与仅采用半胱胺构建的传感器比较,该传感器具有灵敏度高,线性范围宽,操作简单并能实时监测的优点。传感器的检测下限为0.01mIU/mL,在0.06～149.12mIU/mL内有很好的线性关系。A new piezoelectric quartz crystal immunosensor for hCG detection was constructed. The anti - hCG antibody was deposited on the gold electrode＇ s surface of 9 MHz quartz AT - cut crystal by self - assembled cysteamine technique and carbonylic carbon nanotube. Compared with conventional antibody immobilization methods, the sensitivity of the sensor coated by cysteamine and carbonylic carbon nanotube is improved than those by cysteamine only. Under the optimized experimental conditions, the results showed that the low detection limit was 0.01 mIU/mL, and the linear range was 0. 06 - 149.12 mIU/mL.

关 键 词：压电石英晶体 免疫传感器 人绒毛膜促性腺激素 碳纳米管 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]