RP-HPLC法测定垂阴茶糖浆中岩白菜素的含量  

Determination the Content of Bergenin in Chuiyincha Syrups by RP-HPLC

作　　者：张文忠[1] 蒋平[1] 林芳[1] 范耀[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《中国药师》2006年第11期1029-1030,共2页China Pharmacist

摘　　要：目的:测定垂阴茶糖浆中岩白菜素的含量。方法:采用反相高效液相色谱法,色谱柱:VP-ODS(150 mm×4．6 mm,5μm),流动相:甲醇-水(15:85);检测波长:275 nm;流速:1．0 ml·min-1;柱温:室温。结果:岩白菜素的线性范围0．25～2．52μg,r=0．999 9,平均回收率为98．76％,RSD％=1．3(n=6)。结论:该法适用于测定垂阴茶糖策中岩白菜素的含量。Objective： To set up a RP-HPLC method for the determination of content of Bergenin in Chuiyincha syrups. Method： The VP-ODS column（ 150 mm ×4.6 mm,5μm） was used. The mobile phase consisted of methanol-water （ 15： 85）, and the detection wavelength was at 275nm. Result： The linear range of Bergenin was 0.25 - 2.52μg （ r = 0. 999 9 ）. The average recovery was 98.8% ,RSD =1.3% （n = 6）. Conclusion： The method can be used for the determination the content of Bergenin in Chuiyincha syrup.

关 键 词：垂阴茶糖浆 岩白菜素 反相高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]