G-CSF对VP16诱导白血病细胞凋亡及Survivin mRNA表达的调控作用  

G-CSF Regulation of Survivin mRNA Expression and VP16-induced-apoptosis in Leukemia Cells

作　　者：李中东[1] 齐振华[2] 舒义刚[2] 王光平[2] 陈方平[2] 

机构地区：[1]河南省焦作市人民医院血液科,454002 [2]中南大学湘雅医院血液科

出　　处：《天津医药》2006年第10期682-685,共4页Tianjin Medical Journal

摘　　要：目的:探讨粒细胞集落刺激因子(G-CSF)对足叶乙甙(VP16)诱导白血病细胞凋亡以及Survivin基因表达的影响。方法:用HL-60白血病细胞株进行传代培养,通过DNA凝胶电泳和流式细胞术(FCM)检测细胞凋亡,用RT-PCR方法检测Survivin基因表达。结果:VP16可诱导HL-60细胞凋亡,下调Survivin表达;G-CSF可上调SurvivinmRNA表达,与VP16联合应用时,细胞凋亡率低于单独应用VP16组(P<0.01),SurvivinmRNA表达水平高于单用VP16组(P<0.01)。结论:VP16诱导白血病细胞凋亡可能与其抑制Survivin表达有关;G-CSF能抑制VP16的促凋亡作用。Objective： To discuss the effect of G-CSF on survivin expression and VP16 induced apoptosis in leukemia cells. Methods： HL-60 leukemia cells were serial subcultured; DNA gel electrophoresis and FCM were used to make a apoptosis research and RT-PCR was used to make a survivin gene expression research. Results： Apoptosis of HL-60 cells could be induced by VP16 interference accompanied by down-regulation of survivin expression. G-CSF up-regulated survivin mRNA expression, when combined with VP16 apoptotic rate was lower than that of the VP16 group alone （P 〈 0.01） and survivin mRNA expression was higher than that of the VP16 group alone （P 〈 0.01）. Conclusion： Inhibition of survivin expression play an important role in VP16 induced apoptosis in leukemic cells and G-CSF inhibite the effect of VP16-induced-apoptosis.

关 键 词：白血病 细胞凋亡 SURVIVIN 依托泊甙 粒细胞集落刺激因子 

分 类 号：R979.1[医药卫生—药品]