超临界流体CO_2萃取龙胆总苷的工艺研究  

Supercritical fluid extraction of glycosides from Radix Gentianae

作　　者：李莲芳[1] 梁晓原[1] 

机构地区：[1]云南中医学院,云南昆明650200

出　　处：《广东药学院学报》2006年第5期508-509,共2页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的探讨采用超临界流体CO2萃取技术提取龙胆中龙胆总苷的工艺。方法应用超临界流体CO2对龙胆总苷进行提取,在提取过程中加入体积分数为95%的乙醇作为夹带剂,用紫外-可见分光光度法(UV-2450)测定其总苷含量。结果在萃取压力25 MPa,萃取温度50℃,分离压力6 MPa,分离温度55℃的条件下,所得的提取物中总苷量最高。结论通过加夹带剂的方法,应用超临界流体CO2萃取技术可提取出龙胆中极性较大的苷类成分。Objective To establish a method for extracting the glycosides of Radix Gentianae. Methods Supercritical CO2-ethanol was used to extract the glycosides. The content of total glycosides was measured by UV. Temperatures, pressures and concentrations of ethanol （modifier） and the ratio （w/w） of solvent to material were tested in this study. Results Twenty-five MPa of extraction pressure,50 ℃ of extraction temperature,6 MPa of separation pressure and 55℃ of separation temperature, were the most favorable extraction conditions. Conclusion The glycosides could be effectively extracted from Radix Gentianae by supercritical fluid extraction with CO2-ethanol.

关 键 词：超临界流体CO2萃取 龙胆总苷 紫外分光光度法 夹带剂 

分 类 号：TQ461[化学工程—制药化工]