高效液相色谱法测定多西紫杉醇脂质体药物含量及包封率  

Analysis of content and entrapment efficiency of docetaxel in docetaxel liposomes by HPLC

作　　者：汪晓凌[1] 吕竹芬[1] 陈燕忠[1] 谢清春[1] 黄红兵[2] 刘韬[2] 

机构地区：[1]广东药学院药物研究所,广东广州510006 [2]华南肿瘤学国家重点实验室/中山大学附属肿瘤医院,广东广州510080

出　　处：《广东药学院学报》2006年第5期513-515,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省科技攻关项目(2005B30101004)

摘　　要：目的建立测定多西紫杉醇脂质体药物含量及包封率的HPLC法。方法采用Diamonsil C18柱（250mm×4．6mm，5μm）：流动相：甲醇-乙腈-水（体积比35：40：25）；紫外检测波长：230nm。结果在本色谱条件下多西紫杉醇与辅料及溶荆峰分离良好，多西紫杉醇在1．0～50．0μg／mL范围内线性关系良好（r=0．9996，n=7），平均回收率为101．51％，RSD为1．96％。结论该方法准确可靠、简单快速，可用于多西紫杉醇脂质体含量及包封率的测定。Objective To establish an HPLC method for the determination of content and entrapment efficiency of docetaxel in docetaxel liposomes. Methods The analysis was performed on a C18 column （250 mm×4.6 mm,5μm）, The mobile phase consisted of methanol-acetonitrile-water （35： 40： 25） ,and the detection wavelength was 230 nm. Results The linear range for docetaxel was 1.0 - 50.0 μg/mL （r =0. 9996,n = 7）. The average recovery was 101.51% and RSD was 1.96%. Conclusion This method is simple, accurate, sensitive for measurement of content and entrapment efficiency of docetaxel in the liposomes .

关 键 词：多西紫杉醇脂质体 含量测定 包封率 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]