何首乌生根培养因子的优选研究  

OPTIMIZATION OF THE CULTURE CONDITIONS FOR THE ROOTING OF POLYGONUM MULTIFLORUM PLANTLETS

作　　者：陈菊[1] 陈国惠[1] 

机构地区：[1]西南大学农学与生命科学学院,重庆400716

出　　处：《西南农业大学学报（自然科学版）》2006年第5期756-758,共3页Journal of Southwest Agricultural University

基　　金：重庆市自然科学基金资助项目(8024)

摘　　要：以何首乌嫩茎为外植体,采用正交实验设计进行何首乌生根培养的研究。实验结果表明:影响何首乌生根培养因子的作用大小为培养基>IBA>蔗糖>NAA,诱导何首乌组培苗生根的最佳培养基为:1/2 MS+NAA 0.2 mg/L+IBA 1.0 mg/L+30 g/L蔗糖。In an experiment with the orthogonal design, young shoots of Polygonum multiflorum Thunb were cultured on MS or 1/2 MS medium containing different coneentrations of IBA, saceharose and NAA to induce rooting of the explants. Rooting induction was significantly affected by the concentrations of MS and IBA, followed by saeeharose and NAA. The optimum combination of the culture condition was 1/2 MS ＋ NAA0.2 mg/L ＋ IBA 1.0 mg/L ＋ saccharose 30 g/l.

关 键 词：何首乌 组织培养 正交设计 

分 类 号：S567.239[农业科学—中草药栽培]