盐碱土中放线菌分离方法研究  

Study on the isolation methods of actinomycetes from saline-alkaline soils

作　　者：来航线[1] 盛敏[2] 杨保伟[3] 薛泉宏[1] 

机构地区：[1]西北农林科技大学资源与环境学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100 [3]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2006年第10期113-117,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：西北农林科技大学土壤学博士点基金项目(2003)

摘　　要：研究了不同分离培养基及样品预处理(热处理和化学处理)对盐碱土中放线菌分离效果的影响。结果表明:(1)碱土中放线菌的总数、属数和链霉菌类群数极显著地高于盐土;(2)高氏1号琼脂为最佳的基础培养基,精氨酸-甘油琼脂次之;(3)A3培养基(高氏1号琼脂,N aC l 0.5 g/kg,pH 9.0)为碱土的最佳分离培养基;A1培养基(高氏1号琼脂,N aC l 50 g/kg,pH 7.0)为盐土的最佳分离培养基;(4)盐碱土用无菌的20 g/kg腐殖酸溶液浸泡,于40℃下振荡20 m in,可提高放线菌的出菌率并抑制杂菌生长。The effect of basic culture media ,saline content,pH and different pretreatments of saline-alkaline soils on actinomycete isolation was studied. The results showed that the quantity of actinomycetes, genus and streptomyces varieties in alkaline soils was higher than that in saline soils ;Gaoshi No. 1 agar was best among all the basic culture media,next was Arginine-Glycerine agar;The basic culture media holding the moderate pH（pH 9.0） and saline concentration （NaCl 50 g/kg） was good to actinomycete isolation;A3 （NaCl 0. 5 g/kg,pH 9.0） was the best isolation culture media when applying for alkaline soils;A1 （NaCl 50 g/kg,pH 7.0） was the best isolation culture media when applying for saline soils ;It could promote actinomycete development and inhibit the development of other microorganism when the saline-alkaline soil was immersed in HA 20 g/kg solution and vibrated at 40 C for 20 minutes.

关 键 词：盐碱土 放线菌 分离培养基 样品预处理 出菌率 

分 类 号：S154.383[农业科学—土壤学]