甘蔗DNA导入糯玉米自交系后代性状变异的研究  

Study on the Mutant Character of Waxy Maize Inbred Line Offsprings Introducing Sugarcane DNA

作　　者：张慧英[1] 吕平 庾韦花[3] 郝小琴[1] 王邕[4] 

机构地区：[1]广西大学农学院,南宁530005 [2]广西亚热带作物研究所,南宁530001 [3]广西农业科学院生物所,南宁530007 [4]广西大学生命科学与技术学院,南宁530005

出　　处：《玉米科学》2006年第4期32-34,共3页Journal of Maize Sciences

基　　金：广西自然科学基金资助项目(桂科基0236005);广西教育厅资助项目(桂教科研[2002]316)

摘　　要：利用浸种法将外源DNA导入玉米自交系,后代产生了多种多样的变异类型,变异性状十分丰富,主要包括株高、穗位高、叶片数、叶片形状与颜色、叶片的长度与宽度、光合叶面积、百粒重和生育期等。这些变异性状有的具有供体性状,有的产生新的性状,并且在D3代趋向稳定。从D0到D3代连续4代田间性状观察发现,变异植株的株高、穗位高均变矮,叶色浓绿。对D3代稳定株系的可溶性糖含量进行测定分析,结果表明,可溶性糖含量都有所提高。该方法为改良玉米品质,创造玉米新种质提供一条新的途径。With the methods of seed-immersion, the exogenous DNA extracted from Heipi sugarcane was introduced into the maize inbred lines. The progenies appear various mutant types and the mutant characters were very abundant, mainly including the height of plant, the height of fruit,the number of leaf, the color and morphology of leaf, the length and width of leaf, the area of leaf, the weight of 100 grain and the period of growth, etc. Some of the varied characters were similar to donors; some of the varied characters were newly produced,which tended to be stabilized in D3 generation. Continuously observing and counting on field-grow characters from Do to D3 generation, found that the plant height and fruit height were shortened, the color of leaves was greener, compared with the acceptor. Analyzed its soluble sugar content of the stable strain D3, the results indicated that the soluble sugar content was increased. Consequently, it created a new way to develop new maize material for improving quality of maize.

关 键 词：外源DNA 浸种法 玉米自交系 性状变异 

分 类 号：S513.035.3[农业科学—作物学]