人催乳素时间分辨荧光免疫分析检测试剂的研制  

Preparation of Time-Resolved Fluoroimmunoassay Kit for Detection of Human Prolactin

作　　者：吴英松[1] 汤永平 董志宁 徐伟文[1] 李明[1] 

机构地区：[1]南方医科大学生物技术学院,广州510515 [2]广州市达瑞抗体工程技术有限公司,广州510665

出　　处：《热带医学杂志》2006年第10期1061-1063,1087,共4页Journal of Tropical Medicine

基　　金：广州市重大科技攻关项目(No.2004Z1-E4021);广东省科技攻关项目(No.2005B10401045)。

摘　　要：目的建立人血清催乳素的时间分辨荧光免疫分析(TRFIA)方法。方法采用双抗体夹心法建立人血清催乳素TRFIA检测试剂盒,对试剂盒的各项指标进行评价。结果试剂盒的定量范围为16～11000mU/L,灵敏度为1.36mU/L,批内批间的精密度分别为2.6%～7.6%,5.7%～9.0%;与人胎盘泌乳素(hPL)、人生长激素(hGH)、人黄体生成素(hLH)和人促卵泡激素(hFSH)无交叉反应。30份异常血样用本试剂盒与进口的同类试剂盒同时检测,其相关系数为0.997。结论试剂盒各项指标均达到临床检测要求,可替代国外同类产品试剂盒。Objective To prepare a time-resolved fluoroimmunoassay （TRFIA） kit for detection of human prolactin, Method A sandwich TRFIA kit for detection of human prolactin was developed using monoclonal antibody of anti-prolactin, Result The measure range of the kit was 16~11000 mU/L, Its assay sensitivity was 1.36 mU/L, There was no cross reactivity with human placental lactogen, human growth hormone, human luteinizing and human follicle stimulating hormones. The intra- and inter-assay coefficients of variation were 2.6%-7.6% and 5.7%-9.0%, respectively. The correlation coefficient of blood samples detection results between home-made and commercially available PRL-DELFIA （PerKin Elmer） was 0.997, Conclusion The developed prelactin TRFIA kit in this study was a valuable test for clinical application with better sensitivity, specificity, and accuracy.

关 键 词：人催乳素 时间分辨免疫荧光分析(TRFIA) 检测 

分 类 号：R446.6[医药卫生—诊断学]