广东地区丙型肝炎患者的HCV基因分型研究  

Genotyping of Hepatitis C Virus Obtained from Patients in Guangdong Area

作　　者：胡斌[1] 张孝文[2] 程钢[1] 何蕴韶[1] 劳绍贤[3] 

机构地区：[1]中山大学达安基因诊断中心,广州510080 [2]中山大学附属第三医院,广州510630 [3]广州中医药大学脾胃研究所,广州510405

出　　处：《热带医学杂志》2006年第10期1071-1072,1127,共3页Journal of Tropical Medicine

摘　　要：目的了解广东地区慢性丙型肝炎患者的HCV基因分型情况,为丙型肝炎病毒的诊断和预防提供有力依据。方法根据丙型肝炎病毒RNA全序列,在5′-NCR区设计引物,通过套式PCR扩增,特异性产物测序分析并分型。结果检测HCV感染样本58例,扩增出阳性样本47例,共检出3种基因型,分别为1b,2a和4型,其中1b占85.1%,2a占12.8%,4型占2.1%,4型为中国人群中极少报道被检出的罕见型。结论广东地区HCV基因型主要为1b型,其次为2a型,与中国其它地区HCV基因型分布比较一致。Objective To investigate the prevalent of hepatitis C virus （HCV） in Guangdong, and to provide information for the diagnosis and prevention of HCV. Method Based on the full RNA sequence of the HCV, we designed the primers for the domain of 5＇-NCR, and amplified the target sequence using reverse transeription-polymerase chain reaction （RT-PCR）. The genotyping of HCV was determined by the sequencing. Result 58 serum samples from HCV infected patients were examined, 47 samples were found positive with HCV. Three different HCV genotypes namely lb, 2a and 4, were identified. The frequency of distribution of the genotype lb, 2a, and 4 was 85.1%, 12.8%, and 2.1%, respectively. The genotype 4 was rare the Chinese population. Conclusion HCV lb is the major genotype distributed in Guangdong. The prevalent strains of HCV in Guangdong area are similar to HCV found in other regions in China.

关 键 词：丙型肝炎病毒 套式RT—PCR 测序 基因分型 

分 类 号：R698[医药卫生—泌尿科学]