ICAM-1在大鼠胰腺移植缺血再灌注损伤中的作用  

Role of ICAM-1 During Ischemia/Reperfusion Injury of Rat Pancreas Transplantation

作　　者：田青水[1] 朱兴国[1] 金同玉[1] 李德春[1] 陈彦[1] 闫永鑫[1] 

机构地区：[1]连云港市第一人民医院普外科,江苏连云港222000

出　　处：《苏州大学学报（医学版）》2006年第5期744-746,750,共4页Suzhou University Journal of Medical Science

摘　　要：目的探讨ICAM-1在大鼠胰腺移植缺血再灌注损伤中的作用。方法将大鼠随机分为假手术组(对照组)和全胰十二指肠移植组,于再灌注后1 h3、h、6 h取血测定血清淀粉酶水平,并切取胰腺标本进行组织病理学观察及ICAM-1免疫组化染色。结果(1)移植组胰腺组织损伤随再灌注时间的延长而加重,血淀粉酶升高,与中性粒细胞浸润直接相关;而对照组胰腺组织损伤不明显,血淀粉酶不升高。(2)移植组各时段ICAM-1均表达,且随再灌注时间的延长表达逐渐增加,对照组ICAM-1不表达。结论中性粒细胞的活化以及释放出各种蛋白酶和活性因子,是导致胰腺移植缺血再灌注损伤的直接原因。ICAM-1参与胰腺移植缺血再灌注损伤的病理过程。Objective To investigate the role of ICAM-1 during ischemia/reperfusion injury of rat pancreas transplantation. Methods Forty five Sprague-Dawley rats were randomized into the transplanted group and the sham operation group. The transplanted group rats received whole pancreatico- duodenal transplantation. Rats in the sham operation group were anesthetized, laparotomized, resected left kidney and clamped the aorta below left renal vein for the same time with the transplanted group. Pancreas and blood samples were harvested at I hour（ n = 5）, 3 hour（ n = 5） and 6 hour（ n = 5） after releasing the clamp for immunohistochemistry analysis of ICAM-1, histopathology and serum amylase assay. Results （1）The damage of graft pancreas tissue was aggravated gradually and the level of serum amylase was elevated markedly as reperfusion was prolonged. These changes were related to neutrophil infiltration, while these changes were mild in the sham operation group. （2）ICAM-1 were expressed in the transplanted group, but were not expressed in the sham operation. The expression of ICAM-1was augmented gradually as reperfusion was prolonged. Conclusion （ 1 ） It resulted in ischemia/reperfusion injury in pancreas transplantation that neutrophil was activated and various kinds of proteolytic ferment and active factors are released. （2） ICAM-1 may play important roles in ischemia/ reperfusion injury in rat pancreas transplantation.

关 键 词：胰腺移植 ICAM-1 免疫组织化学 缺血再灌注损伤 

分 类 号：R392.4[医药卫生—免疫学]