应用3种抗原的芯片系统检测结核分枝杆菌  

Analyzing Mycobacterium Tuberculosis with Three Antigens Chip System

作　　者：周正康[1] 顾国浩[1] 史进方[1] 李忆[2] 吴兴福[3] 胥萍[3] 钱雪峰[1] 高纯[1] 

机构地区：[1]苏州大学附属第一医院检验科江苏省临床免疫学重点实验室 [2]苏州大学附属第一医院肺科,江苏苏州215006 [3]苏州市第五人民医院,江苏苏州215007

出　　处：《苏州大学学报（医学版）》2006年第5期751-753,共3页Suzhou University Journal of Medical Science

摘　　要：目的探讨3种抗原的芯片系统检测结核分枝杆菌的敏感性及特异性。方法以3种结核抗原的芯片检测系统,对临床确诊的100例肺结核标本及100例非结核标本进行检测。结果芯片检测的敏感度为88%,两项或三项指标同时阳性时,特异度可达100%;芯片检测结核组与非结核组结果有高度显著性差异(P<0.01)。结论结核分枝杆菌3种抗原的芯片敏感性高、特异性强。Objective To discuss the sensitivity and specificity of three antigens chip system in detecting mycobacterium tuberculosis. Method One hundred pulmonary tuberculosis and 100 non-pulmonary tuberculosis serum samples were analyzed respectively with three antigens chip system. Results The chip system sensitivity in detecting mycobacterium tuberculosis was 88%, the specificity achieved to 100 % when two or three items were positive simultaneously. Significant difference existed between pulmonary tuberculosis and non-pulmonary tuberculosis when analyzed with this chip system （ P 〈0.01 ）. Conclusion Three antigens chip system is sensitive, specific, simple, accurate and will be valuable in clinical applications.

关 键 词：结核分枝杆菌 抗原芯片 

分 类 号：R446.52[医药卫生—诊断学]