毛细管区带电泳富集分离烟草生物碱(英文)  

Preconcentration and separation of tobacco alkaloids by capillary zone electrophoresis

作　　者：许庆平[1] 王蕾[1] 何友昭[1] 

机构地区：[1]中国科学技术大学化学系,安徽合肥230026

出　　处：《中国科学技术大学学报》2006年第10期1096-1100,1126,共6页JUSTC

基　　金：National Natural Science Foundation of China (20275035)

摘　　要：采用pH 3.2,360 mmol/L酒石酸缓冲体系,毛细管区带电泳能成功地将烟草生物碱基线分离.与磷酸缓冲液相比,该方法提高了分离度和检测灵敏度.米喔斯明的分离度和检出限分别为1.6和2 ng/mL.此外,还研究和采用了卷烟样品中痕量生物碱的毛细管电泳阳离子选择性耗尽在柱富集方法.Tobacco alkaloids were separated on baseline by capillary zone electrophoresis with a buffer solution of pH 3.2, 360 mmol/L tartaric acid. Cation-selective exhaustive injection, as an on-column preconcentration method, was also investigated for the determination of the alkaloids of cigarette samples. With the proposed method, both the resolution and detection limit of the alkaloids were improved comparing with those in phosphate buffer. The resolution and detection limit of myosmine was 1.6 and 2 ng/mL, respectively.

关 键 词：毛细管区带电泳 酒石酸缓冲液 烟草生物碱 阳离子选择性耗尽注入 

分 类 号：O658[理学—分析化学]