稀土元素铕对掌叶大黄Ri质粒转化根生长及蒽醌类成分的影响  

Effect of Eu^(3+) on Growth and Production of Anthraquinone in Ri Plasmid-Transformed Root and Normal Roots of Rheum palmatum

作　　者：杨世海[1] 陶静[1] 刘晓峰[2] 果德安[3] 郑俊华[3] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林亚泰(集团)股份有限公司,长春130031 [3]北京大学药学院,北京100083

出　　处：《吉林农业大学学报》2006年第5期538-541,共4页Journal of Jilin Agricultural University

基　　金：国家自然科学基金资助项目(39470849)

摘　　要：试验研究了不同质量浓度的稀土元素铕(Eu3+)对掌叶大黄单克隆毛状根DH5c、DH7a和非转化根Nor生物量、蒽醌化合物合成的影响。结果表明:Eu3+浓度对不同类型大黄根的生物量积累作用效果不同。Eu3+对非转化根Nor和转化根DH7a的生物量积累有抑制作用,低浓度的Eu3+对转化根DH5c生物量积累有促进作用。Eu3+浓度对不同类型大黄根的蒽醌产量有不同影响。单克隆毛状根DH7a在Eu3+为0.1 mg/L和0.05 mg/L时蒽醌产量最高。经Eu3+处理的3种类型大黄根中芦荟大黄素和大黄酸高于大黄素、大黄酚和大黄素甲醚。The effect of rare-earth elements Eu^3＋ on biomass and anthraquinone production of two hairy clones DH5c, DH7a and normal root Nor cultures of Rheum pa/matum was evaluated after various concentration of Eu3^＋ was administrated into culture medium. The results showed that the concentration of Eu3^＋ had different effects on biomass accumulation of roots. Eu3^＋ had inhibitive effect on biomass of Nor and DH7a, and low concentration of Eu3^＋ had promotive effect on biomass of DH5c. Concentration of Eu3^＋ had different effects on anthraquinone production of roots. Combination of DH7a and 0.1 mg/L, 0.05 mg/L concentration of Eu3^＋ presented the highest anthraquinone yield. Aloe-emodin and rhein were predominant in five anthraquinones after being treated with rare-earth element Eu^3 ＋ .

关 键 词：铕 掌叶大黄 Ri质粒转化根 葸醌类化合物 

分 类 号：S567.23[农业科学—中草药栽培]