丽春红2R-亮绿法在中枢神经纤维束髓鞘染色中的应用  

Application of ponceau 2R-brilliant green stain for myelin in demonstrating fasciculi of the central nervous system

作　　者：栗卓[1] 吕广明[1] 刘苏[1] 吴辉群[1] 韩笑[1] 季达峰[1] 

机构地区：[1]南通大学医学院人体解剖学教研室神经生物学研究所江苏省神经再生重点实验室,江苏226001

出　　处：《交通医学》2006年第5期494-495,F0004,共3页Medical Journal of Communications

基　　金：国家自然科学基金重点项目(90307013);江苏省神经再生重点实验室开放课题J(SK-04003);江苏省高校自然科学基金项目(05KJB180105);南通市社会发展基金项目(S40052)资助

摘　　要：目的:探索一种中枢神经纤维束髓鞘的染色方法。方法:应用丽春红2R-亮绿双重染色法,对正常SD大鼠脊髓组织进行染色。结果:丽春红2R-亮绿染色后,脊髓白质中神经纤维束轴索、髓鞘呈橙红色,灰质背景呈淡绿色,其中神经元呈绿色。结论:丽春红2R-亮绿法是显示中枢神经纤维束的一种简便、迅速、可靠染色法。Objective： To investigate a myelin stain method for visualizing the fasciculi in the central nervous system（CNS）. Methotis： The spinal cord sections of the adult Sprague-Dawley rats were detected by using Ponceau 2R - brilliant green double stain method. Results： In the Ponceau 2R - brilliant green staining sections, the axons and myelin of fasciculi in the white matter of the spinal cord were stained salmon red, and the gray matter and the neurons were stained green. Conclusions： Ponceau 2R - brilliant green double staining technique is a quick, simple and reliable myelin stain method in demonstrating the fasciculi in the central nervous system.

关 键 词：神经髓鞘 神经纤维束 丽春红2R-亮绿染色法 脊髓 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]