利用rep-PCR研究云南尼泊尔桤木根瘤内Frankia基因多样性  

Genetic Diversity of Frankia Strains in Alnus nepalensis Nodules in Yunnan Revealed by rep-PCR

作　　者：熊智[1] 唐晓萌[1] 代玉梅[1] 张成刚[1] 张忠泽[1] 徐丽华[2] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]云南大学教育部微生物资源重点实验室,昆明650091

出　　处：《应用与环境生物学报》2006年第5期623-627,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点基础研究发展项目(001CB1089)~~

摘　　要：利用rep-PCR指纹技术对云南5个地区的尼泊尔桤木根瘤内Frankia菌基因多样性进行研究,实验发现Frankia菌呈现丰富的基因多样性.所有71个样品被分为11种不同的基因类型.除高黎贡山外,不同地域都有某种基因型占优势,显示Frankia菌基因型与地域有紧密关系.所观察的5个地区中,高黎贡山Frankia菌基因型种类最多,多样性指数最高,基因多样性最为丰富.结合高黎贡山地理资料及其它生物多样性相关研究,推测与高黎贡山尼泊尔桤木共生的Frankia可能作为种储备库,为其它地区的寄主植物提供了祖先菌株.rep-PCR technique was used to study the genetic diversity of Frankia strains in Alnus nepalensis collected from five regions in Yunnan. The fingerprint patterns revealed that Frankia strains had great genetic diversity. 71 samples for this study were collected and divided into eleven genotypes. The genotypes were found dominant in the regions except that of the Gaoligong Mountains. The Frankia in the region of the Gaoligong Mountains was found with most genotypes and highest diversity index. Based on the rep-PCR analysis data, geographic data and other studies on biodiversity, it was concluded that Frankia strains symbiotically associated with A. nepalensis growing in the Gaoligong Mountains were perhaps the storercom offering ancestor strains for host plants growing in other regions.

关 键 词：FRANKIA REP-PCR 尼泊尔桤木 云南 地域 基因多样性 

分 类 号：S792.14[农业科学—林木遗传育种]