饮食诱导肥胖与肥胖抵抗大鼠血清差异蛋白筛选  

THE DIFFERENTIAL SCREENING OF SERUM PROTEINS IN DIET-INDUCED OBESE AND OBESE-RESISTANT RATS

作　　者：赵丹[1] 麻微微[1] 王舒然[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院营养与食品卫生教研室,哈尔滨150081

出　　处：《营养学报》2006年第5期415-417,422,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30300283);黑龙江省高等学校骨干教师创新能力资助项目(No.1053G020)

摘　　要：目的:探讨高脂饲料诱导的肥胖(diet-inducedobesity,DIO)与肥胖抵抗(diet-inducedobesityresistance,DIO-R)大鼠不同易感性的差异及血清中蛋白表达。方法:健康雄性SD大鼠40只,随机分为基础组和高脂饲料组,分别给予基础饲料和高脂饲料5w后,参照Levin等的方法建立动物模型,按照体重增加量排序,位于体重上1/3的大鼠筛选为肥胖大鼠,位于体重下1/3的大鼠筛选为肥胖抵抗大鼠。处死动物,收集血清样品检测各组相关指标的差异,并应用SELDI蛋白质芯片技术检测DIO与DIO-R大鼠血清蛋白的差异表达。结果:DIO大鼠体重﹑体脂比﹑血糖﹑血脂均与DIO-R大鼠有显著性差异。由蛋白质芯片检测图可见,在分子量2～100ku范围内,分子量为7945和9513的蛋白峰在DIO大鼠和DIO-R大鼠血清中存在差异;分子量为6256、6267、4496的蛋白峰在DIO-R大鼠和基础组大鼠血清中存在差异。结论:SD大鼠对高脂饮食诱导的DIO和DIO-R存在易感性差异,应用SELDI蛋白质芯片技术筛选出了DIO大鼠与DIO-R大鼠血清差异蛋白,为以后进行蛋白质分离﹑纯化及鉴定提供依据。Objective： To explore the difference in susceptibility to high-fat diet induced obesity in rats, as well as the changes of serum proteins. Method： Forty male SD rats were divided into basic group and high-fat diet group randomly. After 5w feeding DIO （ diet-induced obesity） and DIO-R（diet-induced obesity resistance） rats were selected according to their body weight gain. The rats were sacrificed and the changes of serum proteins were screened using WCX2 proteomic chips made by American Ciphergen Biosystems. Results： DIO rats were significantly different from DIO-R rats in body weight, body-fat ratio, blood glucose and blood lipids. At the molecular weight range between 2 to 100 ku, the proteins with molecular weight of 7 945 and 9 513 were significantly expressed differently between DIO and DIO-R rats, and the proteins with molecular weight of 4496, 6152, 6267 were significantly expressed differently between DIO-R and control rats （the rats were fed basic diet）. Conclusion： Different susceptibility to DIO or DIO-R was found in SD rats when they were fed high-fat diet. The differentially expressed serum proteins between DIO rats and DIO-R were observed, which might provide the basis for further isolation, purification and identification of these proteins.

关 键 词：肥胖 肥胖抵抗 高脂饲料 蛋白质芯片技术 

分 类 号：R589.2[医药卫生—内分泌]