外源激素和眼柄提取物对罗氏沼虾卵母细胞的离体诱导作用  

IN VITRO INDUCTION EFFECTS OF EXOGENOUS HORMONES AND EYESTALK EXTRACTS ON THE OOCYTES OF MACROBRACHIUM ROSENBERGII

作　　者：赵维信[1] 贾江[1] 安苗 

机构地区：[1]上海水产大学,200090 [2]贵州省水产研究所,贵阳550000

出　　处：《上海水产大学学报》1996年第4期221-225,共5页Journal of Shanghai Fisheries University

摘　　要：使用四种类固醇激素，两种保幼激素类似物（JHA）和眼柄提取物进行罗氏沼虾离体卵巢小块培育，于25℃培育24h后切片并进行光镜观察。发现17α—羟孕酮、孕酮和JHA－ZR515对卵黄发生前期和卵黄发生期卵母细胞卵径增大均有极显著刺激作用（P＜0．01）。高浓度雌二醇对卵黄发生前期卵母细胞卵径增大也有明显刺激作用（P＜0．01）；高浓度JHA－ZR512对成熟前卵巢卵母细胞卵径培大有显著作用（P＜0．05）。蜕皮激素对卵径增大无作用（P＞0．05），眼柄提取物对卵黄发生期卵母细胞则有抑制作用，使卵径明显减小（P＜0．05）。研究结果表明，罗氏沼虾的卵巢发育受孕激素和保幼激素样物质的直接作用。The ovarian fragments of Macrobrachium rosenbergii in vitro incubation with four steroids, two juvenile hormone analogues (JHA) and extracts of eyestalk in have been conducted. At 25℃ for 24h incubation, the diameters of maximum oocytes were measured under the light microscope. 17α- hydroxyprogesterone, progesterone and JHA - ZR515 showed significant stimulation (P<0. 01 ) on oocyte diameter increase at the stages of previtellogenesis and vitellogenesis. High concentration of oestradiol had a significant stimulation (P<0. 01) on oocyte diameter increase at the previtellogenesis stage. Also high concentration of JHA-ZR512 stimulated (P< 0. 05 ) oocyte enlargement at prematuration stage.Ecdysone did not affect oocyte diameter. Extracts of eyestalk showed a inhibition on oocyte at the vitellogenesis stage, oocyte diameter was decreased (P<0. 05). These results showed that the ovarian development of M. rosenbergii was stimulated directly by progestogen and juvenile hormone-like compounds.

关 键 词：罗氏沼虾 卵母细胞 离体培育 激素 

分 类 号：S966.121[农业科学—水产养殖]