烟草漂浮育苗旧苗盘残留物TMV带毒检测  

Detection of TMV in Residue of Tobacco Roots on Tray Using in Float Seedling System

作　　者：刘勇[1] 陈学军[1] 李梅云[1] 夏玉珍[1] 

机构地区：[1]云南省烟草科学研究所,云南玉溪653100

出　　处：《湖南农业大学学报（自然科学版）》2006年第5期502-504,共3页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：云南省烟草公司项目(04A15)

摘　　要：为了建立烟草漂浮育苗旧苗盘残留物中烟草花叶病毒TMV的检测方法,以TMV病苗干根为材料,采用直接研磨法、液氮研磨法和浸泡研磨法制样,效果分别用三抗体夹心法(TAS-ELISA),TMV试纸条和枯斑寄主法检测灵敏度.结果表明,3种方法制备的样品都能用TAS-ELISA和试纸条检测出阳性,且3种制样方法间的差异小;3种制样方法制备的样品都具有侵染力,浸泡研磨法最强,直接研磨法次之,液氮研磨法最弱.TAS-ELISA,TMV试纸条和枯斑寄主接种的检测下限分别为干病根占健康根质量的0.01%,0.10%和0.10%.In order to develop detection methods of Tobacco mosaic virus in residue of tobacco roots on tray using in float seedling system, by using dry roots of TMV-infected tobacco float seedling, three sample prepare methods including direct grinding, liquid nitrogen grinding and dip grinding, and three detection methods sensitivity including TAS-ELISA, TMV-lmunostrip and local lesion assay are evaluated. Samples prepared by three methods can detect TMV positive and show no obvious difference between each other when detected by TAS-ELISA and TMV-Imunostrip, Samples prepared by three methods show TMV effective when inoculated into Nicotiana ghainosa, the local lesion number order is： dip grinding 〉direct grinding 〉liquid nitrogen grinding. The detection threshold of TAS-ELISA, TMV-Imunostrip and local lesion assay are 0.01%, 0.10%, 0.10% dry TMV-infected roots among healthy roots respectively.

关 键 词：漂浮育苗 烟草 根 烟草花叶病毒 检测 

分 类 号：S572[农业科学—烟草工业] S41-30[农业科学—作物学]