人参化感物质对人参愈伤组织生长的影响  

Study on the Influence of Ginseng Allelopathy on Growth of Ginseng Callus

作　　者：陈长宝[1] 许世泉[2] 刘继永[2] 李昌禹[2] 王英平[2] 张连学[1] 

机构地区：[1]吉林省农业大学中药材学院,吉林长春130118 [2]中国农业科学院特产研究所,吉林长春132109

出　　处：《同济大学学报（医学版）》2006年第5期37-38,45,共3页Journal of Tongji University(Medical Science)

基　　金：国家自然科学基金资助课题(30370159)

摘　　要：目的 探讨人参连作障碍的内外在因素作用机制。方法 通过添加人参提取物和根际土壤物质培养愈伤组织方法研究人参化感作用，通过酶联免疫法分析生长激素（IAA）探讨对抑制生长的机制。结果 生晒参和根际土提取物均显著抑制愈伤组织发育，根基土壤提取物抑制高于人参提取物。各种人参愈伤组织内源IAA的含量各处理变化趋势差别明显，根际土壤提取物两部分处理与对照及生晒参处理区别各异，根基土壤处理内源IAA的含量0～20d呈下降形式，对照和生晒参处理，0～7d呈上升趋势，7～20d又逐渐降低。结论 通过生晒参和根际土提取物均显著抑制愈伤组织发育，显示人参植株及其根基土壤提取物均有抑制效应的化感作用，根际土壤抑制高于参根。Objective To cliscuss the internal and external factors＇ functional mechanism of ginseng continuous cropping obstacle. Methods Ginseng allelopathy was studied by adding it＇s extraction and rhizosphere soil material to cultivate callus; The mechanism of IAA in inhibiting the growth was analyzed by enzyme-linked hnmunosorbent assay. Results Dried white ginseng and rhizosphere soil extraction both significantly inhibitted the growth of callus, and rhizosphere soil extraction was more effective. Each disposal of endogenesis IAA in various ginseng callus had an obvious changing trend, and the two factions of rhizosphere soil extraction, CK and dried white ginseng were different. The content of endogenesis IAA treated with rhizosphere soil declined in 0 - 20 days, while CK and dried white ginseng disposals rose in 0 - 7 days, but declined gradually in 7 - 20 days. Conclusion For both dried white ginseng and rhizosphere soil extraction significantly inhibit the growth of callus and it shows that both ginseng and it＇s rhizosphere soil extraction have the inhibitory allelopathy and rhizosphere soil extraction is more effective.

关 键 词：人参化感作用 根际土壤 愈伤组织 生长素 

分 类 号：R285.1[医药卫生—中药学]