胰岛素样生长因子-1对脓毒症大鼠血浆应激激素和胰岛素样生长因子-1受体表达的影响  

Effect of Insulin-like Growth Factor-1 on the Stress Hormone and Insulin-like Growth Factor-1 Receptor Gene Expression and Protein Content in Pyaemia Rats

作　　者：高志光[1] 刘敏丰[1] 秦环龙[1] 沈通一[1] 

机构地区：[1]上海交通大学附属第六人民医院普通外科,上海200233

出　　处：《中国临床营养杂志》2006年第5期303-307,共5页Chinese Journal of Clinical Nutrition

基　　金：上海市卫生发展科技基金(024119039)

摘　　要：目的探讨胰岛素样生长因子-1(IGF-1)对脓毒症大鼠血浆应激激素及骨骼肌IGF-1受体(IGF-1R)蛋白质和mRNA表达的影响。方法选择71只SD大鼠采用盲肠结扎穿孔法建立腹腔感染及颈静脉置管肠外营养(PN)模型后,将存活的30只大鼠随机分为脓毒症组(n=10)、PN组(n=10)及IGF-1组(n=10),另设正常组(n=10)。正常组和脓毒症组给予生理盐水,PN组给予营养支持,IGF-1组为PN+IGF-1静脉注射,连续5天。第6天行颈动脉置管,开腹取门静脉血测定胰岛素、胰高血糖素、肾上腺素、皮质醇和IGF-1水平;免疫组织化学法及实时定量PCR法测定肝脏和骨骼肌IGF-1R蛋白质和mRNA的表达。结果与对照组相比,脓毒症组大鼠血浆胰岛素、胰高血糖素、肾上腺素、皮质醇水平显著升高,IGF-1显著下降(P<0.01);IGF-1组前5项指标较脓毒症组和PN组显著降低,IGF-1则显著升高(P<0.05,P<0.01)。脓毒症组肝脏和骨骼肌IGF-1R蛋白质及mRNA表达较对照组显著增强(P<0.05,P<0.01);IGF-1组的IGF-1R蛋白质及mRNA表达较脓毒症组和PN组显著降低(P<0.01)。结论IGF-1可改善应激激素之间的平衡,降低脓毒血症大鼠IGF-1R在蛋白质和mRNA水平的表达,从而有利于血糖的控制。Objective To investigate the effect of insulin-like growth factor-1 （ IGF-1 ） on the stress hormone, IGF-1 receptor （IGF-1 R） protein and mRNA expression. Methods Totally 71 SD rats with abdominal infection models established by cecal ligation and perforation method were randomly divided into three groups： pyaemic group （ n = 10）, parenteral nutrition （PN） group （n = 10）, and IGF-1 group （ n = 10）. Another 10 SD rats were set as the normal control group （ n = 10）. On the sixth day, one sampling catheter in a single carotid was performed. The vena cava blood was sampled to determine the levels of insulin, glucagon, adrenalin, corticosteroid, and IGF-1. In addition, the expression of the IGF-1 R in liver and muscle were detected by immunohistochemistry and reverse transcription-polymerase chain reaction. Results The levels of plasma insulin, glucagon, adrenalin, and corticosteroid in the IGF-1 group were significantly lower than those in pyaemic group and PN group （ P 〈 0. 05, P 〈 0.01 ） , and IGF-1 level in IGF-1 group was significantly higher than those in of the pyaemic group and PN group （ P 〈 O. 05 ）. The expressions of liver and muscle IGF-1 R protein and mRNA of the pyaemic group and PN group were significantly higher than those in of the normal group （ P 〈 0. 05, P 〈 0. 01 ） , while the expression in the IGF-1 group was significantly lower than those in pyaemic group and PN group （P 〈 0.01 ）. Conclusion IGF-1 can improve the balance among stress hormones, decrease the expression of IGF-1 R at protein and mRNA levels, and thus improve the control of blood glucose.

关 键 词：脓毒症 胰岛素抵抗 胰岛素样生长因子-1 应激激素 胰岛素样生长因子-1受体 

分 类 号：R459.7[医药卫生—急诊医学]