养寿丹对AD模型大鼠脑内β-APP蛋白表达的影响  

Effect of Yangshoudan on the expression of β-APP in rat's brain with Alzheimer's disease

作　　者：蒋灵芝[1] 熊平[2] 

机构地区：[1]深圳大学生命科学学院,深圳518060 [2]华南农业大学资源环境学院,广州510642

出　　处：《深圳大学学报（理工版）》2006年第4期368-372,共5页Journal of Shenzhen University(Science and Engineering)

基　　金：深圳大学科研启动基金资助项目(200627)

摘　　要：大鼠腹腔注射D-半乳糖,并同时注射鹅膏蕈胺酸(IBO)于双侧脑Meynert基底核建立AD动物模型,石杉碱甲作阳性药物对照组,用免疫组织化学染色法检测脑内β-APP蛋白的表达水平.手术阳性对照组、中药大剂量组和中药小剂量组大鼠脑内β-APP表达水平均低于AD模型组(P<0.05).中药剂量越大,β-APP蛋白表达越低.实验结果证明养寿丹可以降低脑内β-APP表达水平.This study is carried out to find out the effect of Yangshoudan on the expression of β-APP in rat＇s brain with Alzheimer＇s disease （AD）. The AD animal model was achieved by intraperitoneal injection of D-galactose and injection with ibotenic acid （IBO） in both sides of the basal Ganglia of meynert in rat brain. Huperzine A was used as positive pharmaceutic control. The expression of β-APP in the brain was detected by immunohistochemical staining. It showed that the expression levels of β-APP in the surgery group, high-dose-yangshoudan treated group and low-dose-yangshoudan treated group were distinctly lower than that in the AD model group. And higher dose of Yangshoudan results in lower expression of β-APP. It indicated that Yangshoudan could down regulate the expression of β-APP in the brain.

关 键 词：养寿丹 中药 脑组织 β-APP蛋白 AD动物模型 大鼠 

分 类 号：R285.5[医药卫生—中药学]