新城疫病毒F48E9株病毒糖蛋白的功能分析  

Functional Analysis of Glycoproteins of Newcastle Disease Virus F 48 E 9 Strain

作　　者：曹殿军[1,2] 刘明[1,2] 王莉林 张莹[1,2] 卢景良 

机构地区：[1]中国农业科学院哈尔滨兽医研究所 [2]中国农业科学院兽医生物技术国家重点实验室

出　　处：《中国兽医学报》1996年第6期534-539,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金;"八五"攀登计划资助项目

摘　　要：对抗新城疫病毒（ＮＤＶ）ＨＮ和Ｆ糖蛋白的单克隆抗体（ＭｃＡｂ）的生物学活性，应用ＥＬＩＳＡ、ＨＩ、ＨＬＩ、Ｗｅｓｔｅｒｎｂｌｏｔ等进行了分析，结果证明，ＮＤＶＦ４８Ｅ９株ＨＮ蛋白除有ＨＡ和ＮＡ功能区外，还有一个促进（启动）融合的功能区。此外，还筛选到４株ＮＤＶ强毒株特异性ＭｃＡｂ。Biological activities of monoclonal antibodies which against Newcastle disease virus HN and F glycoproteins were assayed using the techniques such as ELISA, HI, HLI, and Western blot. A functional area of promoting fusion was found in addition to HA and NA activities in HN protein. Four NDV virulent specific McAbs were identified, providing a basis for differentiation of virulent and avirulent strain of NDV.

关 键 词：新城疫病毒 糖蛋白 单克隆抗体 功能分析 

分 类 号：S852.65[农业科学—基础兽医学]