应用核酸杂交技术检测畜禽衣原体病的研究  

Studies on the detection of Chlamydiosis in Poultry and Animals Using Nucleic Acid Technic

作　　者：赵亚芳[1] 苗镇川[1] 何秀贤 张喜彦[1] 张宝发[1] 

机构地区：[1]内蒙古畜牧科学院

出　　处：《中国兽医杂志》1996年第10期6-7,共2页Chinese Journal of Veterinary Medicine

摘　　要：从纯化山羊流产衣原体颗粒提取ＤＮＡ，用ＤＮＡ限制性内切酶切割，进行酶切图谱分析，与文献报道的图谱对比，证实确系纯的衣原体ＤＮＡ制品。将衣原体ＤＮＡ用光敏生物素标记，制成核酸探针，用斑点杂交法检测衣原体核酸，灵敏度可达１０ｐｇ。又用重组克隆技术将衣原体ＤＮＡ片段克隆于大肠杆菌质粒载体上，筛选出衣原体特异的ＤＮＡ片段制成光生物素探针，可以同样有交效地检测衣原体核酸。用光生物素衣原体探针检测了山羊、绵羊、豚鼠、小白鼠、鸡胚等的衣原体感染病料，结果准确。与间接血凝法检测衣原体感染做了对比试验。Strain of goat abortion of Chlamydia psittaci was purified and its DNA was extracted. Restriction endonuclease digestion indicated that the DNA was purified chromosomal DNA of Chlamydia psittaci. The chromosomal DNA was labelled by pholobiolin, Chlamydia psittaci DNA was detected using blot-hybridization, the sensitivity was 10pg. Recombinant plasmids of Chlamydia psittaci and pUC 18 were labelled by pholobiolin, samples of goat, sheep, guineapig, mice infected with Chlamydia psittaci were detected, comparing to HI indicated that nucleic acid and hybridization was more sensitive and specific.

关 键 词：衣原体 核酸探针 畜禽病 

分 类 号：S855.1[农业科学—临床兽医学] S854.44[农业科学—兽医学]