重组人骨形成蛋白-2聚氰基丙烯酸正丁酯纳米微球缓释系统对骨髓间充质干细胞增殖分化影响的实验研究  

Effects of recombinant human bone morphogenetic-2 polybutylcyanoacrylate nanoparticles on proliferation and differentiation of cultured bone mesenchymal stem cells

作　　者：侯锐[1] 毛天球[1] 陈富林[1] 杨耀武[1] 程晓兵[1] 吴炜[1] 

机构地区：[1]第四军医大学口腔医学院颌面外科,西安710032

出　　处：《口腔医学》2006年第5期339-341,共3页Stomatology

摘　　要：目的明确重组人骨形成蛋白(rhBMP)-2聚氰基丙烯酸正丁酯纳米微球缓释系统对骨髓间充质干细胞(BMSCs)增殖和分化的影响。方法在rhBMP-2纳米微球作用于BMSCs后,采用嗜银蛋白(AgNORs)染色法检测细胞的增殖状况;采用反转录PCR方法,观察细胞中骨钙素、碱性磷酸酶及Ⅰ型胶原mRNA水平表达的改变以反映对细胞分化的影响,并与单纯rhBMP-2作用细胞后的结果进行比较。结果该纳米微球缓释系统能显著促进BMSCs的增殖以及细胞中骨钙素、碱性磷酸酶及Ⅰ型胶原mRNA水平的表达,且均高于单纯rhBMP-2的作用。结论rhBMP-2纳米微球缓释系统促进BMSCs增殖以及向成骨细胞方向分化的作用强于单纯rhBMP-2的作用。Objective To observe the effects of the rhBMP-2 poly butylcyanoacrylate nanoparticles sustained release system on proliferation and differentiation of BMSCs. Methods AgNORs stain was used to evaluate the proliferation of the cells. RT-PCR methods were used to observe the mRNA expressions of osteocalcin, ALP and type Ⅰ collagen, which could reflect differentiation of the cells, in BMSCs by adding the rhBMP-2 nanoparticles or only rhBMP-2. Results Statistical analyses showed that rhBMP-2 nanoparticles could enhance proliferation of BMSCs significantly. And mRNA expressions of the osteocalcin, ALP and type Ⅰ collagen in the rhBMP-2 nanoparticles group were much stronger than those in rhBMP-2 group. Conclusion It was suggested that the rhBMP-2 polybutyl cyanoacrylate nanoparticles sustained release system has significantly higher effects on proliferation of BMSCs and turning the BMSCs into osteoblasts than rhBMP-2.

关 键 词：重组人骨形成蛋白-2 聚氰基丙烯酸正丁酯 纳米微球 骨髓间充质干细胞 增殖 分化 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]