秦川牛和中国荷斯坦牛POU1F1基因多态性研究  

Analysis of POU1F1 Gene Polymorphisms in Qinchuan Cattle and Chinese Holstein Cattle

作　　者：严林俊[1] 刘波[2] 房兴堂[1] 陈宏[1] 张润锋[2] 鲍斌[1] 张海军[1] 

机构地区：[1]徐州师范大学细胞与分子生物学研究所,徐州221116 [2]西北农林科技大学动物科技学院 陕西省农业分子生物学重点实验室,杨凌712100

出　　处：《遗传》2006年第11期1371-1375,共5页Hereditas（Beijing)

基　　金：江苏省高校自然科学基金(编号:03KJD230216);陕西省自然科学基金(编号:2004C122);国家高技术研究发展计划项目(国家863计划)(编号:2003AA243051);西北农林科技大学拔尖人才支持计划项目资助~~

摘　　要：采用PCR-RFLP技术研究了秦川牛(QQ)和中国荷斯坦牛(HC)共计218头个体POU1F1基因的多态性。结果表明:秦川牛及中国荷斯坦牛群体POU1F1-HinfⅠ基因座的451 bp的PCR产物经限制性酶HinfⅠ消化后表现多态,其等位基因A／B频率分别为0．232／0．768、0．132／0．868;两个群体AA、AB和BB 3种基因型的频率分别为0．030／0．403／0．567、0．007／0．251／0．742。在该基因座秦川牛群体处于Hardy-Weinberg平衡状态,中国荷斯坦牛群体处于不平衡状态。它们在该基因座的杂合度、有效等位基因数、Shannon信息熵、多态信息含量分别为0．356／1．553／0．541／0．292、0．229／1．297／0．390／0．203;秦川牛群体的位点杂合度、有效等位基因数、Shannon信息熵、多态信息含量均大于中国荷斯坦牛群体。PCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle （QQ） and Chinese Holstein cattle （HC）. Results demonstrated Hinf Ⅰ polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0,403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy /polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population.

关 键 词：POU1F1 秦川牛 荷斯坦牛 PCR—RFLP 多态性 

分 类 号：S823[农业科学—畜牧学]