难治性肺炎支原体肺炎患儿T细胞活化功能状态的变化  

Changes of Activated T Cell Disfunction in Children with Refractory Mycoplasma Pneumoniae Pneumonia

作　　者：杨勇[1] 王瑜[1] 陈宗波[2] 

机构地区：[1]榆林市第一医院儿科,陕西榆林718000 [2]青岛大学医学院附属医院儿科,山东青岛266003

出　　处：《实用儿科临床杂志》2006年第20期1400-1401,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨难治性肺炎支原体肺炎(MPP)患儿T细胞活化功能状态。方法采用免疫荧光标记和流式细胞仪技术对26例难治性MPP患儿外周血T细胞亚群的表面标记和活化后表面分子的表达进行分析,并以18例健康儿童外周血作为对照。结果与对照组比较,难治性MPP患儿CD4+T细胞、CD4+/CD8+值、CD3+/HLA-DR+活化T细胞和T细胞活化后CD3+/CD25+细胞的表达率均显著降低(P均<0.01);难治性MPP患儿CD8+T细胞的表达率明显高于对照组(P<0.01);而CD3+T细胞的表达率在两组间无显著差异(P>0.05)。结论难治性MPP患儿存在T细胞免疫功能紊乱和T细胞活化功能障碍。Objective To study disfunction of activated T cell from peripheral blood and explore its role on pathogenesis in patients with refractory mycoplasma pneumoniae pneumonia （MPP）. Methods T cell subsets and expression of membrane molecule on activated T cell in peripheral blood were measured in 26 refractory MPP patients by using immunofluorescence and flowcytometry, and compared with those of 18 control healthy children. Results The percentage of CD4^＋ ceil from peripheral blood in refractory MPP children was significantly lower than that in control group （P 〈 0.01 ）, the expressions of CD3^＋/HLA- DR^＋ and CD3^＋/CD25^＋ on T cell were also lower（all P〈0.01）. The percentage of CD8^＋ ceil was significantly higher in refractory MPP children than that in control group （P〈 0.01 ）, and a ratio of CD4^＋/CD8^＋ was disproportion （P〈0.01）, but there was no significant difference in CD3^＋ cell between the patients and control group （P 〉0.05）. Conclusions There are disfunctions of T celt in refractory MPP patients in acute period, and the abnormal T cell activated disfunction may play an important role on pathogenesis.

关 键 词：肺炎 支原体 难治性 T细胞 活化障碍 

分 类 号：R725.6[医药卫生—儿科]