外源SOD和APX基因在转基因烟草中的表达与遗传  

Expression and Heredity of Exogenous SOD or APX Gene in Transgenic Tobacco (Nicotiana tabacum L.)

作　　者：刘飞虎[1] 梁雪妮[2] 周玮[1] 刘明求[1] 

机构地区：[1]云南大学生命科学学院,昆明650091 [2]云南大学成人教育学院,昆明650091

出　　处：《植物生理学通讯》2006年第5期877-881,共5页Plant Physiology Communications

基　　金：云南省人才基金(2000C0001G)

摘　　要：分析转超氧化物歧化酶基因(SOD)或抗坏血酸过氧化物酶基因(APX)烟草及其自交和杂交后代的叶片中超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的结果表明:转基因烟草的SOD和POD活性在终花期最强,不同叶位叶中SOD活性差异不明显,POD活性以下部叶为最高;转基因烟草的SOD或POD活性显著高于近等基因的非转基因品系。杂交后代(F1、F2)的SOD活性能保持稳定,略高于亲本;自交后代(S1～S3)与自交亲本的SOD和POD活性相当。In this experiment, superoxide dismutase （SOD） and/or ascorbate peroxidase （APX） activities in leaves of SOD- or APX-gene transformed tobacco （Nicotiana tabacum L.） plants and their self-bred and hybridization progenies were analyzed. The transgenic tobacco had highest SOD and peroxidase （POD） activities at the end-bloom stage; no significant difference in SOD activity was observed in leaves at upper, middle and base parts of a plant, but the base leaves had the highest POD activity. The transgenic tobacco had SOD and POD activities remarkably higher than the non-transgenic tobacco. The SOD activities in hybrid progenies （F1 and F2） maintained steady and were a little more than parental plants. While the SOD or POD activities of self-bred progenies （S1-S3） and their parents were similar.

关 键 词：转基因烟草 超氧化物歧化酶 抗坏血酸过氧化物酶 外源基因 基因表达 

分 类 号：Q943.2[生物学—植物学]