豹皮樟黄酮类提取物抗氧化作用机制的研究  

Research on the mechanism of antioxidation of flavoniods from leaves of Litsea coreana

作　　者：叶辉[1] 金丽琴[1] 郁建平[2] 吴建章[3] 

机构地区：[1]温州医学院基础医学院生化教研室 [2]贵州大学生化营养研究所,贵州贵阳550025 [3]温州医学院药学院,浙江温州325035

出　　处：《温州医学院学报》2006年第5期424-427,共4页Journal of Wenzhou Medical College

摘　　要：目的:探讨豹皮樟黄酮类提取物抗氧化作用的机制。方法:运用二苯代苦味肼基自由基法对山奈酚、山奈酚-3-O-β-D-葡萄糖甙、槲皮素-3-O-β-D-葡萄糖甙三种贵州豹皮樟(Litsea Coreana)黄酮类提取物进行了抗氧化活性研究;同时,还采用了组织匀浆丙二醛比色法测定了以上三种化合物对大鼠离体心、脑、肝、肾四种组织脂质过氧化的影响。结果:无论是采用二苯代苦味肼基自由基法还是组织匀浆丙二醛比色法,以上三种化合物均具有明显的抗氧化活性,并呈量效关系。且抗氧化活性为:山奈酚>槲皮素-3-O-β-D-葡萄糖甙>山奈酚-3-O-β-D-葡萄糖甙;另外,在组织匀浆丙二醛比色法中,以上三种物质对SD大白鼠四种离体组织丙二醛抑制率为:肾组织>心组织>肝组织>脑组织。结论:豹皮樟黄酮类提取物抗氧化作用可能是通过清除自由基而实现的。Objective：To explore the mechanism Litsea coreana. Methods： The antioxidiantion of of antioxidation of flavoniods from leaves of three flavoniods from leaves of Litsea Coreana was tested using the methods of DPPH and tissue homogenate MDA. Results：These compounds had obvious effects of antioxidiantion and inhibition with dose effect relationship, and their effect was Kaempferol 〉 Querctin-3-0- β -D-glucopyranoside 〉 Kaempferol-3-0- β -D-glucopyranoside; Moreorer, compared with the inhibitory rate of tissue homogenate MDA of heart,brain,liver and kidney, it was found that their effect was kidney〉heart〉liver〉brain. Conclusion： The flavoniods from leaves of Litsea coreana have antioxidation through elimination free radical in vivo. antioxidation.

关 键 词：豹皮樟 山奈酚 山奈酚-3-0-β—D-葡萄糖甙 槲皮素-3-0-β—D-葡萄糖甙 抗氧化 

分 类 号：R285.5[医药卫生—中药学]