藏红花提取液对慢性高眼压兔眼玻璃体谷氨酸浓度的影响  

Effect of Crocus sativus extracti on the concentration of glutamic acid in the vitreous body in rabbits with chronic ocular hypertension

作　　者：杨新光[1] 张强 于敬妮[1] 廉江平[3] 雷爱军[1] 

机构地区：[1]中国陕西省西安市第四医院眼科,710002 [2]中国山东省烟台市中医医院眼科,264002 [3]中国陕西省人民医院药剂科,3710068

出　　处：《国际眼科杂志》2006年第5期1025-1026,共2页International Eye Science

基　　金：中国陕西省科技创新项目(No.2004K10-G7)~~

摘　　要：目的:探讨慢性高眼压后兔眼玻璃体谷氨酸浓度的变化情况及藏红花提取液对谷氨酸浓度的抑制作用。方法:将新西兰白兔20只随机分为对照组、高眼压组、治疗Ⅰ组和治疗Ⅱ组,每组均5兔5眼。高眼压组和治疗Ⅰ、Ⅱ组兔眼前房注入3g/L复方卡波姆溶液制作成慢性高眼压模型。治疗Ⅰ、Ⅱ组兔每日耳缘静脉推注50g/L藏红花提取液。4wk后利用高效液相色谱仪测定各组兔眼玻璃体中谷氨酸的浓度。结果:高眼压组与对照组、治疗组兔眼玻璃体谷氨酸浓度有显著性差异(P<0.01),且治疗Ⅰ组与治疗Ⅱ组有显著性差异(P<0.01)。结论:慢性高眼压可导致玻璃体谷氨酸浓度升高,对视网膜产生损伤;藏红花提取液可显著降低玻璃体中谷氨酸的浓度,对这种视网膜功能损害起到保护作用。AIM： To explore the concentration of glutamic acid in vitreous body of rabbits with chronic ocular hypertension （OHT） and the inhibition effect of extract of Crocus sativus on the concentration of glutamic acid. METHODS： Twenty rabbits were randomly divided into control group, OHT group and two treated groups, with 5 rabbits in each group （5 eyes）. The 3g/L carbomer into anterior chamber of the OHT group and the two treated groups. Rabbits in two treated groups were treated with extract of Crocus sativus every day. High-performance liquid chromatography （HPLC） was utilized to measure the concentration of glutamic acid in vitreous body.RESULTS： The concentration of glutamic acid in vitreous body of rabbits in the OHT group were significantly higher than those in control group and treated group （P〈0.01）. Significant difference appeared between the treated group I and treated group Ⅱ （P〈0.01）. CONCLUSION： Higher concentration of vitreous glutamate damaging ritina was induced by OHT. Extract of Crocus sativus can significantly inhibit the vitreous glutamate concentration.

关 键 词：慢性高眼压 玻璃体 谷氨酸 高效液相色谱仪 藏红花 

分 类 号：R285[医药卫生—中药学]