Herceptin和9-顺式视黄酸对MDA-MB-453乳腺癌细胞协同抑制作用的研究  

Synergistic effects of Herceptin and 9-cis-RA on MDA-MB-453 breast cancer cells

作　　者：柴雅明[1] 糜漫天[1] 郎海滨[1] 

机构地区：[1]第三军医大学军事预防医学院营养与食品卫生学教研室,重庆400038

出　　处：《第三军医大学学报》2006年第22期2234-2236,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30300285)~~

摘　　要：目的 探讨Herceptin和9-顺式视黄酸联合用药对MDA—MB-453乳腺癌细胞的协同抑制效应。方法用Herceptin、9-顺式视黄酸单独和联合处理MDA—MB-453细胞24～72h，用MTT法观察其对乳腺癌细胞的增殖抑制效应，通过流式细胞仪法检测乳腺癌细胞周期相的分布，用TUNEL法检测细胞凋亡的情况。结果 Herceptin和9-顺式视黄酸联合作用组较对照和单独作用组能够显著抑制HER2／neu阳性乳腺癌细胞的增殖活性，并将其细胞周期进程阻遏于G0／G1期，同时还能有效诱导其发生凋亡。结论 Herceptin和9-顺式视黄酸联合用药可有效协同抑制HER2／neu阳性乳腺癌细胞的增殖活性。Objective To investigate the synergistic effects of Herceptin and 9-cis-RA on the proliferation, cell cycle and apoptosis of HER2-positive breast cancer cells. Methods MDA-MB-453 breast cancer cells were treated with 5 μg/ml Herceptin or 1 μmoL/L 9-cis-RA or both for 24, 48 or 72 h. The proliferation of MDA-MB-453 breast cancer cells was determined by MTT, the pro-apoptotic effects were detected by TUNEL, the cell cycle phase was detected by FCM. Results As compared with control group, Herceptin and 9-cis-RA synergistically inhibited the proliferation of MDA-MB-453 breast cancer cells, and the percentage of G0/G1 cells increased after treatment with Herceptin and 9-cis-RA for 72 h. Simultaneously, Herceptin and 9- cis-RA synergistically induced the apoptosis of MDA-MB-453 cells. Conclusion Herceptin and 9-cis-RA could synergistically and effectively inhibit MDA-MB453 breast cancer cells by blocking their cell cycle progression and inducing their apoptosis.

关 键 词：HERCEPTIN 9-顺式视黄酸 HER2 细胞周期 细胞凋亡 MDA—MB-453细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R737.9[医药卫生—基础医学]