急性肝损伤中肝细胞胀亡与肿瘤坏死因子-α的关系  

Relationship between oncosis and TNF-α in acute hepatic injury

作　　者：李荣萍[1] 任成山[1] 李宜辉 

机构地区：[1]第三军医大学新桥医院消化内科,重庆400037

出　　处：《第三军医大学学报》2006年第22期2237-2239,共3页Journal of Third Military Medical University

基　　金：重庆市科委攻关项目(2005AC5127)~~

摘　　要：目的探讨急性肝损伤中肝细胞胀亡与肝组织肿瘤坏死因子-α(TNF-α)的关系。方法应用D-氨基半乳糖(D-GalN)诱导大鼠急性肝损伤模型,同时设立正常对照组,于不同时相检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)含量,肝组织中TNF-αmRNA表达,观察肝细胞胀亡情况,计算胀亡指数(oncosis index,OI)。结果与正常对照组比较,致伤后大鼠血清ALT、AST明显升高(P<0·05),肝组织中TNF-αmRNA表达增强,细胞胀亡增多,OI值增高。结论急性肝损伤中存在细胞胀亡,其发生与TNF-α的表达有一定关系。Objective To investigate the correlation of oncosis and TNF-α in D-galactosamine （D-GalN） induced acute hepatic injury. Methods Acute hepatic injury model was induced by D-GalN in 24 SD rats, and 12 rats served as control. At 4, 8, 12, 16, 20, 24 h after intraperitoneal injection of D-GalN, the levels of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） in serum, and the tumor necrosis factor-α （TNF-α） in liver tissue were measured. The pathological changes of liver and the oncosis of hepatocytes were observed by TEM. Results In acute hepatic injury, the levels of ALT, AST, TNF-α mRNA, OI were higher than that of control group （P 〈 0.05） , and OI increased with the severity and course of acute hepatic injury. Conclusion Oncosis occurs in acute hepatic injury in rats and its mechanism might be related to the TNF-α expression.

关 键 词：D-氨基半乳糖 肝损伤 急性 肿瘤坏死因子-Α 胀亡 肝细胞 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R341[医药卫生—基础医学]