鸵鸟新城疫病毒分子流行病学研究  

Molecular epidemiological studies on newcastle disease virus strains isolated from ostriches

作　　者：尹燕博[1] 龚振华[2] 司微[3] 蒋金书[4] 崔尚金[3] 高齐瑜[4] 

机构地区：[1]山东省莱阳农学院 动物科技学院,山东青岛266109 [2]中国动物卫生与流行病学中心,山东青岛266032 [3]中国农业科学院哈尔滨兽医研究所 兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [4]中国农业大学动物医学院,北京100094

出　　处：《中国预防兽医学报》2006年第6期705-711,共7页Chinese Journal of Preventive Veterinary Medicine

摘　　要：应用RT-PCR、基因序列测定和分析以及鸡胚平均死亡时间(MDT)等方法,对1997～2000年间从中国北方分离的10株鸵鸟源新城疫病毒进行了F-糖蛋白基因序列测定、系统进化分析、基因分型和毒力测定。结果显示：10株鸵鸟源新城疫病毒分属Ⅱ、Ⅲ、Ⅵ、Ⅶ四个基因型。除1株为中发型毒株,其他均为速发型毒株；分析表明,引起鸵鸟发病的新城疫毒株有多种不同的来源。10 NDV strains were isolated from ostriches from different areas in northern of China between 1997-2000. Assessment of the virulence by MDT, RT-PCR and sequence analysis of F glycoprotein gene were used to compare the characteristic properties of the 10 strains, and the phylogenetic tree was obtained, 10 NDV strains isolated from ostriches were belong to four of Ⅱ, Ⅲ, Ⅳ, Ⅶ genotictypes separately. The assessment virulence of the 10 strains showed that 9 were velogenic, and 1 were mesogenic. The results indicated there were many different infectious sources of NDV strains causing the disease in ostrich.

关 键 词：鸵鸟 NDV 毒力测定 基因序列 基因型 

分 类 号：S852.65[农业科学—基础兽医学]