高效液相色谱法测定人血浆中盐酸替扎尼定含量  

Determination of Tizanidine Hydrochloride concentration in human serum by HPLC

作　　者：陈锋杰[1] 张成志[1] 李岑[1] 何海霞[2] 周远大[2] 杨辉[2] 

机构地区：[1]重庆医科大学附属第一医院药剂科,重庆400016 [2]重庆医科大学附属第一医院临床药理研究室,重庆400016

出　　处：《实用医院临床杂志》2006年第6期85-86,共2页Practical Journal of Clinical Medicine

摘　　要：目的建立用高效液相色谱法快速检测盐酸替扎尼定血药浓度的方法。方法取血浆加1mol／L．NaOH．混匀，加氯仿提取吹干，用流动相复溶进样；色谱柱SUNTEK KromasiL C85μm粒径，150×4．6mm；色谱条件：A：乙睛=100：25[A：水-甲酸-氨水（28％）：100：5：10（V／V，pH8．50）]；流速1．0m1／min；紫外检测波长318nm，内标物为雷尼替丁。结果盐酸替扎尼定的保留时间为6．9min，雷尼替丁的保留时间为8．2min，检测的线性范围0．3867～49．5000ng／ml（r=0．9982）方法回收率大于90．78％（n=5）日内RSD=7．74％，日间RSD=3．79％（n：5）。结论本方法简便快速，定量准确，适用于盐酸替扎尼定人体临床药代动力学和生物利用度的研究.Objective To develop a specific HPLC method for measurement of Tizanidine Hydrochloride in human serum. Methods The plasma protein was mixed with 1 mol/L NaOH. Chloroform was used to extract the materials in the serum. The mobile phase was used as solvention. The chromatography conditions were： SUNTEK Kromasil C18 5μm column, A： acetonitrile = 100：25 （ A： water-Formic Acid-Concentrated Ammonia Solution （ 28%）} = 100：5 ： 10 （ V/V, pH 8.50 ）） as mobile phase at 1.0 ml · min^-1. Detection wavelength was 318 nm. Ranitidine was the internal standard. Results The retention time of Tizanidine Hydrochloride and internal standard were 6.9 min and 8.2 min respectively. A good linearity was shown in the concentration rang of 0. 3867 - 49. 5000 ng/ml （ r = 0.9982 ） The recovery was more than 90.78%. The intra-day RSD was 7.74% and the inter-day RSD was 3.79% （ n = 5）. Conclusion This method is simple, rapid and accurate, and eligible for the study of pharmacokinetic and bioavailability of Tizanidine Hydrochloride.

关 键 词：盐酸替扎尼定 高效液相色谱法 血药浓度 

分 类 号：R284.1[医药卫生—中药学]