姜黄素对K562细胞TopoI作用的研究  

Study on the effect of curcumin on TopoI of K562 cell

作　　者：吴秋玲[1] 陈燕[1] 陈卫华[1] 何静[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液病研究所,武汉430022

出　　处：《临床血液学杂志》2006年第6期352-354,共3页Journal of Clinical Hematology

基　　金：国家自然科学基金资助项目(No:30472267)

摘　　要：目的:观察姜黄素对白血病细胞株K562细胞拓扑异构酶TopoI表达的影响。方法:流式细胞术及RT-PCR法分析姜黄素作用K562细胞后TopoⅠ蛋白及mRNA含量的变化。结果:TopoI在K562细胞中高表达,TopoI蛋白的平均荧光强度为84.31±4.26,显著高于阴性对照组3.82±0.17(P<0.01);流式细胞术及RT-PCR法均提示姜黄素能显著抑制TopoⅠ的表达,25μmol/L姜黄素能抑制K562细胞TopoI蛋白的表达超过一半以上,并且随着剂量的增加,姜黄素的抑制作用逐步增强。结论:姜黄素可通过抑制TopoI的表达来抑制K562细胞的增殖,是一种新的TopoI抑制剂,具有良好的临床应用前景。Objective,To investigate the effect of curcumin on topoismerase I of leukemia K562 cells. Method： FCM and RT-PCR were used to analyze the change of Topoi mRNA and protein. Result; Topoi was highly expressed in K562 cells. Mean fluorescence intensity of Topoi protein in blank group （84.31±4.26） was significant- ly higher than that in control group （3.82±0. 17, P 〈0. 001）. Curcumin can significantly inhibit the expression of Topo I by FCM and RT-PCR analysis. 25 μmol/L curcumin can inhibit the expression of Topoi. The inhibitory effect of curcumin increased with increasing dosage of curcumin. Conclusion;Curcumin can inhibit the proliferation of K562 cell through inhibiting the expression of Topoi. Curcumin is a new Topoi inhibitor and has a good clinical application prospect.

关 键 词：姜黄素 K562细胞 拓扑异构酶Ⅰ 

分 类 号：R97[医药卫生—药品]